HDAC10 gene in-situ hybridization detection kit, detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problem of inability to simulate the function of full-length proteins, and achieve the effects of high sensitivity, strong specificity and convenient operation.

Inactive Publication Date: 2015-06-17
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using a mutant of HDAC10 lacking histone deacetylase activity, the researchers confirmed that it cannot mimic the function of the full-length protein

Method used

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  • HDAC10 gene in-situ hybridization detection kit, detection method and application thereof
  • HDAC10 gene in-situ hybridization detection kit, detection method and application thereof
  • HDAC10 gene in-situ hybridization detection kit, detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] An in situ hybridization detection kit for HDAC10 gene, comprising hybridization probes, markers and synergists, wherein the hybridization probe sequence is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0047] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0049] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0050] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0052] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0053] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0054] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0055] Chromogen B ...

Embodiment 2

[0097] A kind of HDAC10 gene in situ hybridization detection method and its kit application

[0098] 1. Specimen processing

[0099] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation medium, then slowly add 3 ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation medium (blood: lymphocyte separation medium = 1:1.5), 2000r / min Centrifuge for 10 min;

[0100] 2. Draw the white blood cells in the middle layer to another centrifuge tube, and then add about twice the amount of 1× buffer to this tube , mix well, centrifuge at 1500g / min for 10 min;

[0101] 3. Discard the supernatant. Add about twice the amount of 1× buffer to the pellet , mix well, centrifuge at 1500g / min for 10 min;

[0102] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide and pushed to dry naturally. (Hospitals with conditions ...

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Abstract

The invention relates to a HDAC10 (Histone Deacetylase 10) gene mRNA in-situ hybridization detection kit, which comprises a hybridization probe and a marker; wherein the sequence of the hybridization probe is represented by the SEQ ID No. AF426160. The invention further provides a HDAC10 gene in-situ hybridization detection method. Besides, the invention also provides an application of the kit in preparation of drugs for treating cervical cancer. The provided kit has the characteristics of high sensitivity and strong specificity; and the detection method has the advantages of simple and convenient operation and can be commonly used and promoted in district hospitals and better hospitals.

Description

technical field [0001] The invention relates to a kit, in particular to an in situ hybridization detection kit of HDAC10 gene and its detection method and application. Background technique [0002] Cervical cancer is a common gynecological malignancy. The occurrence of such important events as invasion and distant metastasis affects the prognosis and treatment of cervical cancer. In the very early stages of cervical cancer, more than 90 percent of women survive at least 5 years after diagnosis. The prospect of invasion or metastasis of advanced cervical cancer is significantly poorer. Fewer than 20 percent of women with stage IV cervical cancer survive 5 years or more. Some cervical cancer cells can metastasize through the lymph nodes or blood circulation and form secondary cancers. They most commonly spread to the liver, lungs, and bones. Therefore, understanding the molecular and cellular mechanisms of cell invasion and metastasis is of great significance for developi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福张玉丽裘建英裘霖
Owner NATUREGEN BIOTECH SHANGHAI
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