Rare cell automatic capture equipment

Abstract

The invention provides a rare cell automatic capture device which can automatically realize functions of sample feeding, reagent feeding, cell detection and the like by taking a microfluidic chip technique as a core. The cell capture efficiency is high and excessive human intervention in the cell detection process is reduced, so that the detection accuracy is improved and the detection cost is lowered. In addition, an injector is arranged on a fixed frame, and after the fixed frame moves, the syringe needle of the injector can be aligned with the sample injection port, so that the determined sample can be automatically fed to a microfluidic chip. Moreover, after a liquid-moving machine moves, a reagent in a kit can be automatically absorbed by virtue of piston movement of an injection pump and the reagent is automatically fed to the microfluidic chip. The rare cell automatic capture device is simple in structure, convenient to operate and low in using cost. In addition, a fluorescence imaging device is arranged to further determine whether the captured cell is a rare cell or not by combining fluorescence imaging and multiple markers, so that the cell detection analyzing efficiency is better improved.

Description

technical field [0001] The invention relates to the field of biological and medical detection, in particular to an automatic capture device for rare cells. Background technique [0002] Circulating Tumor Cells (CTCs) in human peripheral blood are a type of rare cells that are rare in number but have important clinical significance. Under certain conditions, it can develop into tumor metastatic lesions. In general, circulating tumor cells indicate the presence of tumors in humans and possible metastasis. Since more than 90% of clinical cancer deaths are caused by metastasis, and circulating tumor cells provide a source of tumor metastatic lesions in the human body other than tumor lesions, therefore, capturing and detecting circulating tumor cells from blood is becoming more and more important. draw people's attention. [0003] However, the amount of circulating tumor cells in peripheral blood is very small, and every 10 mL of blood may only contain a few to dozens of circ...

AI Technical Summary

Problems solved by technology

[0006] However, the existing circulating tumor cell detection equipment is usually based on magnetic ball separation technology. Not only is the capture efficiency of circulating tumor cells low, but also the operation of the equipment is cumbersome and complicated. More importantly, these equipment and technologies have not yet realized the separation of circulating tumor cells. The automatic control of the detection process, that is, the automatic sampling of blood test samples and reagents, automatic cell capture and identification analysis, thus, there is too much human intervention in the detection of circulating tumor cells, which makes the detection accuracy of circulating tumor cells Low, detection cost is too high

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