Saccharopolyspora spinosa high-pleocidin-yield engineering strain and application thereof

A technology of Saccharopolyspora spinosa and spinosyn, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low yield of spinosyn

Active Publication Date: 2015-10-14
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

[0003] At present, the international research on the synthesis mechanism of spinosyn is still in its infancy, and the yield of spinosyn in wild-type S. spinosa strains is extremely low, so how to increase its fermentation yield is a big problem to be solved urgently

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  • Saccharopolyspora spinosa high-pleocidin-yield engineering strain and application thereof
  • Saccharopolyspora spinosa high-pleocidin-yield engineering strain and application thereof
  • Saccharopolyspora spinosa high-pleocidin-yield engineering strain and application thereof

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Embodiment Construction

[0027] The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0028] The high-yield engineering strain of S. spinosa spinosad spinosyn of the present embodiment, that is, S. spinosa spinosa S.sp-△pepA, Saccharopolyspora spinosa S.sp-△pepA was deposited in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on June 8, 2015, and the strain preservation number is CCTCC NO: M 2015362.

[0029] ①Specific medium formula and culture conditions

[0030] (1) S. spinosa Seed activation uses SP-2 medium: glucose 9 g / L, tryptone soybean broth 30 g / L, yeast extract 3 g / L, magnesium sulfate 2 g / L. Inoculate the monoclonal or 1% strain preservation solution into a 250 mL shake flask filled with 20 mL SP-2, and incubate at 30°C and 300 r / min for 48-72 h;

[0031] (2) Transplant using tryptone soy broth (TSB) medium: tryptone soy broth 30 g / L; inoculate 1% bacteri...

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Abstract

The invention relates to a Saccharopolyspora spinosa high-pleocidin-yield engineering strain and application thereof. The Saccharopolyspora spinosa high-pleocidin-yield engineering strain is Saccharopolyspora spinosa S.sp-DELTApepA which is collected by China Center for Type Culture Collection on June 8th, 2015, and the culture collection number is CCTCC NO:M2015362. The pleocidin yield of the Saccharopolyspora spinosa S.sp-DELTApepA is enhanced to 137% of the initial strain.

Description

technical field [0001] The present invention relates to a spinosad high-yielding engineering strain of Saccharopolyspora spinosa and its application. Genetic engineering technology is used to block the expression of leucyl aminopeptidase homologous gene in Saccharopolyspora spinosa to obtain a high-yield spinosad strain S .sp-△pepA. Background technique [0002] Spinosyn and its derivatives have both high-efficiency insecticidal activity and green and safe characteristics, and have become a hot spot in the field of biological insecticide research in recent years. However, the extremely low fermentation yield of wild strains hinders the further development of spinosad. research problem. Spinosad depolarizes insect nerve cells by binding to nicotinic acetylcholine receptors, causing hyperactivation of the central nervous system, resulting in non-functional muscle contraction, failure and paralysis, so it has rapid contact and ingestion toxicity to insects. Spinosad has a wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12P19/62C12R1/01
Inventor 夏立秋杨燕丁学知胡胜标
Owner HUNAN NORMAL UNIVERSITY
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