Kit for detecting calprotectin in excrement

A technology of calprotectin and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of cumbersome operation, long detection time, inconvenient movement of detection equipment, etc., and achieve simple operation, reliable performance and low cost Effect

Inactive Publication Date: 2015-12-23
SICHUAN ORIENTER BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method used to detect calprotectin is mainly enzyme-linked immunosorbent assay (ELISA). Although the ELISA method has high sensitivity, the operation is cumbersome, the detection time is relatively long, and the detection equipment is large and not easy to move. Therefore, a Easy-to-operate and rapid assay method to detect calprotectin content

Method used

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  • Kit for detecting calprotectin in excrement
  • Kit for detecting calprotectin in excrement

Examples

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Embodiment

[0029] Such as figure 1 As shown, a kit for detecting calprotectin in feces, including a PVC rubber sheet 1, a nitrocellulose membrane 2 arranged on the upper surface of the PVC rubber sheet, an absorbent pad 3, a colored particle / fluorescent particle conjugate pad 4, a sample Pad 5, and detection line 6 and quality control line 7 arranged on the upper surface of the nitrocellulose membrane.

[0030] Specifically, the nitrocellulose membrane is located in the middle of the PVC rubber sheet; the water-absorbing pad is located at one end of the PVC rubber sheet, and one end of the water-absorbing pad is lapped on the upper surface of the nitrocellulose membrane; the colored particles / fluorescent particles are combined The object pad is located at the other end of the PVC rubber plate, and one end of the colored particle / fluorescent particle conjugate pad is lapped on the upper surface of the nitrocellulose membrane; the sample pad is located at the end of the other end of the PV...

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Abstract

The invention discloses a kit for detecting calprotectin in excrement. The kit comprises a PVC (Poly Vinyl Chloride) rubber plate, a nitrocellulose membrane, a water-absorbing pad, a colored particle/fluorescent particle compound pad, a sample pad, a detecting line and a quality control line, wherein the nitrocellulose membrane, the water-absorbing pad, the colored particle/fluorescent particle compound pad and the sample pad are arranged on the upper surface of the PVC rubber plate, and the detecting line and the quality control line are arranged on the upper surface of the nitrocellulose membrane. The kit for detecting calprotectin in excrement is used as a dynamic testing kit for domestic or medical institutions and is used for conveniently detecting the content of calprotectin in excrement; when the detecting sensitivity is 15mu g/g, the detecting time is only 3-5 minutes; the operation is simple; the performance is reliable; the cost is low; the sensitivity is high; the detecting time is short; the domestic and overseas blank is filled up.

Description

technical field [0001] The invention relates to a kit for detecting calprotectin in feces and a preparation method thereof. Background technique [0002] Calprotectin is a calcium and zinc binding protein with a molecular weight of 36kD, which is composed of two heavy chains with a molecular weight of 14kD and a light chain with a molecular weight of 8kD. Each chain can bind two Ca 2+ , so that it has the characteristics of heat resistance and enhanced hydrolysis, and its protein structure is a complex composed of two subunits S-100A8 and S-100A9. It is distributed in human granulocytes, monocytes, epithelial cells, keratinocytes and various tissues and body fluids, and is a protective protein. [0003] Calprotectin is an acute inflammatory marker derived from calcium-containing proteins of neutrophils and macrophages. Its expression is tissue- or cell-specific. It is a heterozygous calcium-binding protein with protease Active, and has the ability to chelate zinc ions and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/065
Inventor 陈功台
Owner SICHUAN ORIENTER BIOLOGICAL TECH
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