Specific primers and typing method of class I mhc gene for detection of anti-virus potential of Chinese alligator

A typing method and anti-viral technology, applied in DNA/RNA fragments, recombinant DNA technology, etc.
CN105349535BActive Publication Date: 2017-06-30ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2017-06-30

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Abstract

The invention relates to a single-locus specific primer of I-class main tissue compatibility compound gene sequence for amplified Alligator sinensis antiviral potention detection and a typing method. A specific sequence of the primer is shown as SEQ ID NO.1-SEQ ID NO.8. The amplified primer has stable single-locus specific amplifying capability in Alligator sinensis populations, a product obtained by amplifying the amplified primer can be used for genotyping of different individuals through subsequent single strand conformation polymorphism (SSCP) experiments, evaluation of I-class MHC gene polymorphism of Alligator sinensis individuals in the populations can be realized, potential, of the Alligator sinensis individuals, in resisting viral diseases can be detected, and important reference can be provided for new population founder selection, wildness reintroduction individual selection and artificial matching in Alligator sinensis protection work.
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Description

technical field

[0001] The invention discloses multiple pairs of single-site specific primers and a typing method for amplifying a class I major histocompatibility complex (MHC) gene sequence for detecting the antiviral potential of Chinese alligator. Background technique

[0002] Alligator sinensis (Alligator sinensis, Fauvel) belongs to Reptilia, Crocodiles, Sinensis, Alligator subfamily, Fauvel, is an ancient animal, originated in the Triassic period 230 million years ago, known as The "living fossil" among reptiles is a rare and endangered animal endemic to my country and a first-class national key protected animal (1972). It is listed in Appendix I of the "Convention on International Trade in Endangered Animals and Plants" (CITES), and is also recognized by the International Union for Conservation of Nature. (IUCN) classified as critically endangered species, is one of the most threatened and endangered species among extant crocodilians. At present, there are only about ...

Claims

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