Sequence siRNA-136 achieving targeted inhibition of mouse interleukin-17A gene

A technology of interleukin and mice, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., to achieve the effect of curing diseases

Inactive Publication Date: 2016-03-23
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on the targeted inhibition of IL-17A expression in mice based on shRNA interference technology, and the relevant siRNA target sequences are worthy of further exploration and application

Method used

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  • Sequence siRNA-136 achieving targeted inhibition of mouse interleukin-17A gene
  • Sequence siRNA-136 achieving targeted inhibition of mouse interleukin-17A gene
  • Sequence siRNA-136 achieving targeted inhibition of mouse interleukin-17A gene

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Experimental program
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Embodiment 1

[0022] The present invention is described below in conjunction with embodiment.

[0023] 1. Sequence design:

[0024] The siRNA interference sequence is designed for the mIL-17A gene for the synthesis of shRNA, and the siRNA-136 gene sequence is SEQ.No.1.

[0025] 2. Construction of mIL-17A interference vector:

[0026] 2.1 OligoDNA design and synthesis:

[0027] The loop structure in the shRNA template uses TTCAAGAGA to avoid the formation of a termination signal, and the shRNA transcription termination sequence adopts the T6 structure. CACC is added to the 5' end of the sense strand template, which is complementary to the sticky end formed after digestion with BbsI; GATCC is added to the 5' end of the antisense strand template, which is complementary to the sticky end formed after digestion with BamHI; if the shRNA first If two bases are not G, add a G after CACC. The mouse IL-17A gene shRNA oligonucleotide sequence (shRNA-136oligoDNA) is shown in Table 1. The design and...

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Abstract

The invention discloses a sequence siRNA-136 achieving targeted inhibition of a mouse interleukin-17A gene. The sequence number of siRNA-136 is SEQ.No.1. The sequence siRNA-136 achieving targeted inhibition of the mouse interleukin-17A gene has the advantages that it is shown through related experimental results that the sequence siRNA-136 can inhibit expression of the mouse interleukin-17A gene; meanwhile, it is prompted through the related experimental results that the sequence siRNA-136 can be used for inhibiting pathological damage caused by expression of the mouse interleukin-17A gene and can adjust expression of related cell factors, and the purpose of curing a disease is achieved.

Description

technical field [0001] The invention relates to a siRNA-136 sequence targeting to inhibit mouse interleukin 17A. Background technique [0002] Interleukin-17A (interleukin-17A, IL-17A) belongs to the IL-17 cytokine family and is an important pro-inflammatory cytokine discovered in recent years. The IL-17 family currently has 6 members, namely: IL-17A~F, among which IL-17A is commonly called IL-17, which is mainly secreted by Th17 cells. After the IL-17 receptor recognizes the corresponding ligand, it recruits Act1 / CIKS [NFκB activator1, nuclear factor NFκB activator 1, also known as TRAF3 action protein 2 (TRAF3IP2), also known as CIKS (ConnectiontoIKK-complexandSAPK)] to phosphorylate it , activate TRAF6 to transmit signals downstream, regulate the expression of inflammatory molecules and chemotactic molecules, such as: IL-4, IL-5, IL-6, IL-9, IL-13, TGF-β, CXCL1, CCL2, CCL7, Expression of CCL20 and C-reactive protein, etc. IL-17A has a pro-inflammatory effect, induces t...

Claims

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Application Information

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IPC IPC(8): C12N15/113
Inventor 贾万忠刘聪暖娄忠子闫鸿斌李立付宝权李文卉张念章
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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