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Construction of double-gene co-expression plasmid pEgr-IL18-B7.2 and application of double-gene co-expression plasmid pEgr-IL18-B7.2 to radiation-combined tumor resisting

A technology of pegr-il18-b7.2 and double genes, which can be used in anti-tumor drugs, wave energy or particle radiation treatment materials, gene therapy, etc., can solve the problem of weak effect

Inactive Publication Date: 2016-03-23
JILIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Practice shows that a single gene therapy is weak

Method used

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  • Construction of double-gene co-expression plasmid pEgr-IL18-B7.2 and application of double-gene co-expression plasmid pEgr-IL18-B7.2 to radiation-combined tumor resisting
  • Construction of double-gene co-expression plasmid pEgr-IL18-B7.2 and application of double-gene co-expression plasmid pEgr-IL18-B7.2 to radiation-combined tumor resisting
  • Construction of double-gene co-expression plasmid pEgr-IL18-B7.2 and application of double-gene co-expression plasmid pEgr-IL18-B7.2 to radiation-combined tumor resisting

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Embodiment Construction

[0028] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are Some, but not all, embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0029] 1. Materials and methods

[0030] 1. Main reagents and equipment

[0031] 1.1 Reagents

[0032]

[0033]

[0034] 1.2 Equipment

[0035]

[0036] 2. Instrument

[0037]

[0038]

[0039] 3. Plasmids and strains

[0040] The double-gene co-expression plasmid pIRES1neo was purchased from BD Company, pMD18-T, pcDNA3.1(+), pGL3-Egr1-...

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Abstract

The invention provides construction of a double-gene co-expression plasmid pEgr-IL18-B7.2 and an application of the double-gene co-expression plasmid pEgr-IL18-B7.2 to radiation-combined tumor resisting. According to the tumor gene-radiotherapy theory and the radiation induction expression characteristics of Egr-1 genes, the double-gene co-expression plasmid pEgr-IL18-B7.2 is constructed on the basis of B7.2 genes of a clone mouse. IL18 genes with the anti-tumor effect and costimulatory-molecule B7-2 genes are transferred into tumor cells through double-gene carriers; the construction and the application further prove that low-dosage radiation can induce radiation-sensitive promoters, and therefore the finer-control effect is achieved on multi-gene expression; radiation-induced double-gene expression shows a certain time-interval and dose-effect rule. According to a test, the constructed double-gene plasmid is further injected into a tumor body of a B16 melanoma transplanted to the mouse, local radiotherapy is carried out in cooperation, and the tumor inhibiting effect more remarkable than that of pure radiotherapy is shown, and preliminary discussion is made for possible mechanisms in the immunology aspect.

Description

technical field [0001] The invention relates to the technical field of tumor gene therapy, in particular to the construction of a pEgr-IL18-B7.2 double-gene co-expression plasmid and its combined radiation anti-tumor application. Background technique [0002] Malignant tumors are one of the main causes of threats to human health in contemporary times. At present, radiotherapy is still one of the main methods for clinical treatment of tumors. With the continuous development of radiation physics and radiation biology, the curative effect of radiation therapy has been greatly improved in the past few decades. However, radiotherapy still has its own shortcomings and difficulties, such as tumor recurrence and metastasis after radiotherapy, and damage to normal tissues caused by radiotherapy, which pose a threat to the survival of cancer patients. The occurrence and development mechanism of malignant tumors is very complicated. It is a multi-factor, multi-link, multi-stage and m...

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Application Information

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IPC IPC(8): C12N15/66A61K48/00A61K41/00A61P35/00
Inventor 杨建征郭杰王铁君于雷边丽闫文星任晓俊宋新灵
Owner JILIN UNIV
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