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Targeting polypeptide-fluorescent magnetic nanometer complex, preparation method and applications thereof

A magnetic nanometer and peptide-targeting technology, applied in chemical instruments and methods, luminescent materials, animal cells, etc., to reduce pain and economic burden, easy to prepare, and improve the quality of life

Active Publication Date: 2016-06-01
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still no definite conclusion about the cause of tumor
However, there is no such composite nano-functional material that can quickly, accurately and conveniently identify and screen lung cancer stem cells.

Method used

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  • Targeting polypeptide-fluorescent magnetic nanometer complex, preparation method and applications thereof
  • Targeting polypeptide-fluorescent magnetic nanometer complex, preparation method and applications thereof
  • Targeting polypeptide-fluorescent magnetic nanometer complex, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Synthesis of magnetic nanoparticles: 1.25g of ferrous chloride tetrahydrate was fully dissolved in 7.75mL of deionized water, 6.25mL of ammonia water was added dropwise while stirring, the stirring was continued for 10min, and 5mL of polyethyleneimine (0.54g) was added to fully After mixing, pour it into a 50mL polytetrafluoroethylene-lined reactor, react at 134°C for 3 hours at 2 atmospheres, cool naturally to room temperature, collect the black precipitate by magnetic separation, and wash it thoroughly with deionized water. The product is magnetic nano particle.

[0032] (2) Synthesis of fluorescent magnetic nanoparticles: take the magnetic nanoparticles in step (1), add 0.11mg of Cy5-N-hydroxysuccinimide ester (Cy5-NHS-Esters) per mg of magnetic nanoparticles and mix in 1mLDMSO Shake at room temperature in the dark for 12 hours, collect the black precipitate by magnetic separation, and wash with DMSO to obtain fluorescent magnetic nanoparticles.

[0033] (3) Syn...

Embodiment 2

[0035] (1) Synthesis of magnetic nanoparticles: Dissolve 0.5g ferrous chloride tetrahydrate in 13mL deionized water, add 1.5mL ammonia water dropwise while stirring, continue stirring for 10min, add 0.2mL polyethyleneimine and mix well, Pour it into a 50mL polytetrafluoroethylene-lined reactor, react at 120°C for 3 hours at 2 atmospheres, cool naturally to room temperature, collect the black precipitate by magnetic separation, and wash it thoroughly with deionized water. The product is magnetic nanoparticles.

[0036] (2) Synthesis of fluorescent magnetic nanoparticles: take the magnetic nanoparticles in step (1), add 0.11mg of Cy5-N-hydroxysuccinimide ester (Cy5-NHS-Esters) per mg of magnetic nanoparticles and mix in 1mLDMSO Shake at room temperature in the dark for 3 hours, collect the black precipitate by magnetic separation, wash with DMSO, and obtain fluorescent magnetic nanoparticles.

[0037] (3) Synthesis of targeted polypeptide-fluorescent magnetic nanocomposites: tak...

Embodiment 3

[0039] (1) Synthesis of magnetic nanoparticles: 1.5g of ferrous chloride tetrahydrate was fully dissolved in 7mL of deionized water, and 7mL of ammonia water was added dropwise while stirring, and the stirring was continued for 15min. React in a 50mL polytetrafluoroethylene-lined reactor at 160°C for 5 hours at 2 atmospheres, cool down to room temperature naturally, collect the black precipitate by magnetic separation, wash it thoroughly with deionized water, and the product is magnetic nanoparticles.

[0040](2) Synthesis of fluorescent magnetic nanoparticles: take the magnetic nanoparticles in step (1), add 0.11mg of Cy5-N-hydroxysuccinimide ester (Cy5-NHS-Esters) per mg of magnetic nanoparticles and mix in 1mLDMSO Shake at room temperature in the dark for 24 hours, collect the black precipitate by magnetic separation, and wash with DMSO to obtain fluorescent magnetic nanoparticles.

[0041] (3) Synthesis of targeting polypeptide-fluorescent magnetic nanocomposites: take 60m...

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Abstract

The present invention discloses a targeting polypeptide-fluorescent magnetic nanometer complex, a preparation method and applications thereof. According to the present invention, magnetic nanoparticles are adopted as the base, a fluorescent molecule is adopted as a display material, and the polypeptide of stem cells capable of targeting lung cancers is modified to construct the targeting polypeptide-fluorescent magnetic nanometer complex; and the targeting polypeptide-fluorescent magnetic nanometer complex has characteristics of fluorescent tracing capability, magnetic resonance developing capability and specific targeting capability, can efficiently and precisely identify, label, sort and enrich lung tumor stem cells, especially H460 lung tumor stem cells, can be used for monitoring of lung cancer metastasis and treatment effects, provides basis and assurance for lung cancer treatment, substantially reduce the suffering and the economic burden of patients, improve the life quality of patients, is easy to prepare, and has low cost.

Description

technical field [0001] The invention relates to a multifunctional targeting material for tumor diagnosis and treatment, in particular to a targeting polypeptide-fluorescent magnetic nanocomposite and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] Tumor has always been one of the most important threats to human health, and it is the main clinical cause of death in many countries, which is also the biggest dilemma facing the world. At present, for most tumor treatments, only by early detection and diagnosis can effective treatment of tumors be achieved. With the development of science and technology and the advancement of medical treatment, the early diagnosis of tumors mainly depends on tumor tissue-specific biomarkers. Through the combination of biomarkers and tumor cells, tumors are identified, so as to achieve the purpose of detection. So far, there is still no definite conclusion about the cause of tumor. ...

Claims

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Application Information

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IPC IPC(8): A61K49/06A61K49/00C12N5/095C09K11/06
Inventor 朱毅敏周旋濮科峰陈丽莎李春林张能攀
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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