Calibration standard solution configuration device and calibration method for tissue oxygen detector
A technology for calibrating standards and configuring devices, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high requirements of blood gas analyzers and complicated configuration process, and achieve the effects of simple calibration process, wide simulation range and high accuracy
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[0031] Embodiment: A tissue oxygen detector calibration standard solution configuration device of this embodiment, such as figure 1As shown, it includes whole blood feed container 1, fat emulsion feed container 2, buffer solution feed container 3, feed quantitative pump 4, feed solenoid valve 5, raw material U-shaped mixing channel 6, oxygenation container 7, deoxygenation Container 8, intake valve 9, high-pressure oxygen cylinder 10, high-pressure nitrogen cylinder 11, discharge quantitative pump 12, discharge solenoid valve 13, discharge U-shaped mixing channel 14, controller 15, computer 16, discharge liquid container 17 and magnetic stirrer 18.
[0032] The 3 feeding containers are respectively connected to pipelines, and the 3 pipelines are merged into one pipeline through a feed quantitative pump and a feed solenoid valve, and then branched into two pipelines through a U-shaped mixing channel for raw materials. Connect the oxygenation and deoxygenation vessels separatel...
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[0047] The specific implementation is as follows:
[0048] (1), select the raw material solution
[0049] The raw material solution for configuring the liquid tissue model is prepared by mixing whole blood of normal people, scattering medium and buffer solution. The whole blood of normal people is the absorption medium, and the fat emulsion is the scattering medium. The role of the buffer is to keep the pH of the tissue model within the range of normal human blood pH (7.35-7.45) and isotonic with blood cells. The formula of the buffer is as follows: 20gK is dissolved in every 1000ml buffer 2 HPO 4 ·3H 2 O and 2.9gNaH 2 PO 4 2H 2 O, the rest is deionized distilled water. According to the calculation, the pH value of the buffer solution is stable at 7.4 at room temperature; meanwhile, its osmolarity is 0.3 mol / L, which is isotonic with human blood.
[0050] Table 1 lists the formulations of several commonly used liquid tissue models, and the absorption coefficient μ of ...
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