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Tissue culture formula for Euonymus phellomanus and culture method

A technique for tying a winged euonymus and a cultivating method, which is applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve the problems of low reproduction coefficient and the like, and achieve the effects of high reproduction coefficient, meeting the needs of landscaping, and stable shape.

Inactive Publication Date: 2016-07-13
NINGXIA HUANGDA BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used propagation methods of Euonymus bolus are seed propagation and cutting seedlings, which are limited by seasons and have a low reproduction coefficient.

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0037] The present invention will be further described below in conjunction with specific embodiment:

[0038] 1. Preparation of the formula of Euonymus bolus

[0039] 1.1 Preparation and storage of medium mother solution

[0040] MS medium contains nearly 30 kinds of nutritional components. In order to avoid weighing these dozens of components every time the medium is prepared, the various components in the medium can be weighed separately according to the integer multiple of the original amount. Into a concentrated solution, this concentrated solution is called the culture medium mother solution.

[0041] Mother liquor ⅠNH 4 NO 3 33g / L, KNO 3 38g / L, MgSO 4 ·7H 2 O7.4g / L, KH 2 PO 4 3.4g / L, mother liquor II CaCl 2 2H 2 O8.8g / L, mother liquor ⅢH 3 BO 3 620mg / L, MnSO 4 ·H 2 O2140mg / L, ZnSO 4 ·7H 2 O860mg / L, KI83mg / L, mother liquor ⅣNa 2 MnO 4 2H 2 O250mg / L, CuSO 4 ·5H 2 O25mg / L, CoCl 2 ·6H 2 O25mg / L, mother liquor ⅤNa 2 ·EDTA3.73g / L, FeSO 4 ·7H 2 O2.78g...

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Abstract

The invention discloses a tissue culture formula for Euonymus phellomanus and a culture method. A sterile culture system is obtained by disinfecting explants, then tissue culture seedlings are subjected to subculture multiplication and growth and rooting are screened and regulated through matching of different kinds of exogenous hormones with different concentrations for screening and regulation, and the optimum explant and culture medium formula are determined finally and are enabled to adapt to industrial production. With adoption of the tissue culture formula and the method, the Euonymus phellomanus can be propagated massively, the landscaping requirement is met, the shapes of produced seedlings are stable, the production cycle is short, and large-scale rapid production can be realized. The tissue culture formula and the method are not limited by seasons, rapid multiplication is realized, the propagation coefficient is high, excellent features of a female parent can be inherited completely, and a problem is solved for factory production of tissue culture.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a tissue culture formula and a culture method of Euonymus bolata. Background technique [0002] Euonymus is a deciduous shrub belonging to the family Euonymus, also known as ghost arrow feathers, about 4 meters high, with nearly four-edged branches, hard and straight, with 2-4 cork wings, taupe, opposite leaves, long leaves Oval or slightly elliptic oblanceolate, purple flowers, forming cymes, with 2-3 branches, 7-15 flowers, fruit capsule with 4 edges, obround heart-shaped, 7-9 mm long, diameter About 1 cm, pink. Born in forest margins and riparian thickets, above 2000 meters above sea level. Its wood is dense, white and tough. It can be used to make bows, sticks, and wooden nails. The branches can be used for medicine. It is called "ghost elm" in traditional Chinese medicine. It helps blood circulation and has the effect of reducing swelling. It can be used as a fol...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李根生
Owner NINGXIA HUANGDA BIOLOGICAL TECH CO LTD
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