Extraction method for amentoflavone in Selaginella tamariscina

A technology of double flavonoids and an extraction method, which is applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills, inability to meet rapid testing, complex pharmacopoeia operating processes, etc., and achieves high precision, reduced operating skills requirements, and time consumption. short effect

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The pharmacopoeia method has a complicated operation process, requires high operating skills of operators, and at the same time, the detection speed is slow, which cannot meet the purpose of rapid detection in industrial applications

Method used

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  • Extraction method for amentoflavone in Selaginella tamariscina
  • Extraction method for amentoflavone in Selaginella tamariscina
  • Extraction method for amentoflavone in Selaginella tamariscina

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Embodiment 1

[0021] Crush Selaginella through a pulverizer, pass through a No. 3 sieve, weigh about 0.2g, accurately weigh it, mix it with 1g of diatomaceous earth, set aside, transfer it to the ASE5ml extraction pool with a filter membrane in advance, and then add an appropriate amount of diatomaceous earth , shake gently to make it at the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, transfer the extract to a 25ml volumetric flask, dilute to the mark with methanol, centrifuge at 15000r / min for 3min, take the supernatant, and enter it into HPLC for determination.

[0022] The extraction conditions (see table 1) are:

[0023] Table 1

[0024] Extraction cell volume (ml)

5

extraction solvent

Methanol

pressure (psi)

1500

temperature(℃)

110

Static extraction time (min)

5

Static cycle times

3

Flushing volume (%)

80

Purge time (s)

60

[0025] Anal...

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Abstract

The invention discloses an extraction method for amentoflavone in Selaginella tamariscina. The method comprises the following steps: step 1, crushing Selaginella tamariscina and then sieving the crushed Selaginella tamariscina; step 2, weighing 0.2 part by weight of Selaginella tamariscina powder, uniformly mixing the Selaginella tamariscina powder with 1 part by weight of diatomite and standing the obtained mixture for subsequent usage; and step 3, adding a proper amount of the mixture obtained in the step 2 into an extraction pool equipped with a filtering membrane in advance until the diatomite reaches the mouth of the extraction pool, covering a cover of the extraction pool and carrying out static extraction, wherein extract is obtained after completion of extraction, and extraction conditions are that methanol is used as an extraction solvent, extraction temperature is 110 DEG C, static extraction time is 5 min, a flushing volume is 80%, and three static cycles are carried out. The extraction method for amentoflavone in Selaginella tamariscina is simple to operate and short in consumed time and lowers requirements on operation skills of the skilled.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for extracting biflavonoids from Selaginella spicosa. Background technique [0002] In 2010, the Pharmacopoeia recorded the method for extracting the biflavones from Selaginella spica: Take about 0.2g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol precisely, and weigh it. Heat to reflux for 5 hours, let cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product. The chromatographic conditions are as follows: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.1% phosphoric acid solution is used as mobile phase B, and gradient elution is carried out according to different mobile phase ratios; the detection wavelength is 330nm. The number of theoretical plates should not b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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