A method for in vitro directional screening and identification of acetochlor-resistant peanuts

A technology of directional screening and acetochlor, applied in the field of directional screening and identification of peanuts in vitro, can solve the problems of consuming manpower, material resources, and financial resources, and achieve the effects of improving survival rate, saving manpower, and convenient operation

Active Publication Date: 2018-07-13
QINGDAO AGRI UNIV
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Problems solved by technology

[0006] The invention provides a method for in vitro directional screening and identification of acetochlor-resistant peanuts, which can solve the problem that peanut transgenic plants or in vitro mutagenic acetochlor-resistant mutants must be regenerated, domesticated, transplanted and survived in the field before screening. and identification, thus consuming a lot of manpower, material resources, financial resources and other issues

Method used

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Embodiment 1

[0034] A method for in vitro directional screening and identification of acetochlor-resistant peanuts according to the present invention specifically includes the following steps:

[0035] 1. Preparation of culture medium.

[0036] 1) Preparation of somatic embryo induction medium: select MS medium as the basic medium, add 2,4-D to this MS medium to prepare somatic embryo induction medium, the concentration of 2,4-D is 5~15mg / L . In this case, the somatic embryo induction medium was MS+10mg / L2,4-D+30g / L sucrose+8g / L agar, and the pH of the somatic embryo induction medium was adjusted to 5.8. The culture is carried out under the condition that the temperature of the culture room is 24-26°C, the light intensity is 2000-3000 Lx, and the daily light is 12-14 hours, so as to induce the formation of somatic embryos.

[0037] (2) Preparation of mutagenesis medium: Add 3-4 mg / L Pingyangmycin to the somatic embryo induction medium to form a mutagenesis medium. In this case, the muta...

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Abstract

The invention provides a method for in-vitro oriented screening and verification of acetochlor resistant bodies for peanuts. The method mainly comprises the steps that oriented screening for acetochlor resistant bodies is carried out on peanut explants cultured and obtained after induced mutation on a culture medium with acetochlor added; four weeks later, survival explants are transferred to a recovery growth culture medium to be cultured; regenerated plants are obtained through screening, leaves of the regenerated plants are taken and put into an acetochlor solution, and verification of acetochlor resistance is carried out. According to the method, acetochlor resistant bodies are screened and verified in an oriented mode through in-vitro culture, operation is convenient, limitation of seasons is broken through, and a great amount of manpower and a great quantity of material resources and financial resources can be saved.

Description

technical field [0001] The invention relates to a method for in vitro directional screening and identification of peanuts, in particular to a method for in vitro directional screening and identification of acetochlor-resistant peanuts. Background technique [0002] Peanut is one of the important oil crops and economic crops in my country. Peanut is a drought-resistant, barren-resistant and adaptable crop, which is widely planted all over the world. With the continuous development of science and technology, the agricultural labor force is gradually transferred to the city, agricultural mechanization gradually replaces manual work, and herbicides replace manual weeding. Acetochlor is a widely used herbicide. The application on peanuts is spraying after sowing and before emergence of peanuts. Spraying acetochlor after emergence and during the growth period will kill peanut seedlings. However, during the growth period of peanuts, weeds will continue to germinate and grow, espe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G2/30A01G22/40A01G7/06
CPCA01G2/30A01G7/06A01G22/00A01H4/008
Inventor 禹山林王晶珊乔利仙隋炯明杨庆利
Owner QINGDAO AGRI UNIV
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