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A kind of botanical acaricide and preparation method thereof

A kind of source acaricide and plant-based technology, applied in the field of plant-source acaricide and its preparation, can solve the problems of insufficient plant resources and failure to achieve further results, and achieve abundant plant resources, good development and application prospects, and environmental protection. friendly effect

Active Publication Date: 2019-07-30
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, often due to insufficient plant resources, many studies have failed to achieve further results

Method used

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  • A kind of botanical acaricide and preparation method thereof
  • A kind of botanical acaricide and preparation method thereof
  • A kind of botanical acaricide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of the extract of Elymus chinensis: collect the above-ground parts (1 kg) of fresh Elymus chinensis plant, dry in the shade, and pulverize. Add 10 times the volume of ethanol 10L, ultrasonically extract for 30 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain the extract, and dry it in a ventilated and dark place to obtain 85 g of crude extract of Elymus pachycarpa.

[0028] Dissolve the crude extract with a small amount of water, and use macroporous resin column chromatography for preliminary separation and purification. Use 20% methanol aqueous solution to elute 2L of chromatographic column to wash away the highly polar sugar and protein; then use 80% methanol aqueous solution to elute 3L, collect 80% of the eluate, concentrate under reduced pressure, and obtain epsilon Grass extract, 76g, yield 7.6%.

[0029] 2. Preparation of different active parts: Take 20 g of the Elymus chinensis extra...

Embodiment 2

[0032] 1. Preparation of the extract of Elymus chinensis: collect the above-ground parts (1 kg) of fresh Elymus chinensis plant, dry in the shade, and pulverize. Add 20 times the volume of methanol 20L, ultrasonically extract for 20 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain an extract, and dry it in a ventilated and dark place to obtain 80 g of crude extract of Elymus pachycarpa.

[0033] Dissolve the crude extract with a small amount of water, and use macroporous resin column chromatography for preliminary separation and purification. Use 20% methanol aqueous solution to elute 2L of chromatographic column to wash away the highly polar sugar and protein; then use 80% methanol aqueous solution to elute 3L, collect 80% of the eluate, concentrate under reduced pressure, and obtain epsilon Grass extract, 70g, yield 7.0%.

[0034] 2. Preparation of different active parts: Take 20 g of the Elymus chinensis extra...

Embodiment 3

[0037] 1. Preparation of the extract of Elymus chinensis: collect fresh aerial parts (1 kg) of the Elymus chinensis plant, dry in the shade, and pulverize. Add 30 times the volume of ethanol 30L, ultrasonically extract for 30 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain an extract, and dry it in a ventilated and dark place to obtain 98 g of crude extract of Elymus acaria.

[0038] Dissolve the crude extract with a small amount of water, and use macroporous resin column chromatography for preliminary separation and purification. Use 20% methanol aqueous solution to elute 2L of chromatographic column to wash away the highly polar sugar and protein; then use 80% methanol aqueous solution to elute 3L, collect 80% of the eluent, concentrate under reduced pressure, and obtain epsilon Grass extract, 86g, yield 8.6%.

[0039]2. Preparation of different active parts: Take 20 g of the Elymus chinensis extract prepared ...

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Abstract

The invention discloses a botanical acaricide. The main ingredient of the botanical acaricide is elymus nutans extract. The elymus nutans botanical pesticide provided by the invention not only has obvious contact action on agricultural spider mites and does not cause pesticide resistance easily, but also is environmentally friendly, and is safe for non-target organisms such as human bodies and livestock. The elymus nutans is high in cold resistance and drought resistance, is wildly distributed in Tibet, Northwest, North and the like of China, is an important constituent part of grasslands and grassy marshlands, and becomes unique secondary metabolites in the long-term evolution process. At present, in high, cold and wet regions of China, abundant elymus nutans is cultivated and is mainly used for establishing artificial grasslands and pasturable grasslands, and resources are quite abundant. The preparation technology is simple and speedy, the mite killing effect is obvious, raw materials are low in cost and easy to obtain, and therefore, the botanical acaricide has good development and application prospect and economic value.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a botanical acaricide and a preparation method thereof. Background technique [0002] Mites belong to the class Arachnida and the subclass Acari. There are about 300,000 to 500,000 species in the world, and they are the second largest biological group in the animal kingdom after the class Insecta. The herbivorous agricultural mites are found on crops all over the world, and the damage is becoming more and more serious. Agricultural pest mites are species that continuously invade temporary habitats and are highly adaptable to short-lived living environments. They are typical R-type pests due to their small size, strong reproductive capacity, and short lifespan and cycle of each generation. They destroy the normal physiological functions of plants, cause leaf drop, bud drop, and fruit drop, which can cause yield reduction, weaken the tree vigor, and cause...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/44A01P7/02
CPCA01N65/00A01N65/44A01N2300/00
Inventor 刘权高坤彭爱风
Owner LANZHOU UNIVERSITY
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