An efficient and accurate method for detecting melatonin content in Paeoniae officinalis

A technology of melatonin and peony, applied in the accurate detection of melatonin content in peony, high-efficiency field, can solve the problem of not being found, and achieve the effect of simple operation, strong specificity, accurate and reliable results

Active Publication Date: 2018-07-10
YANGZHOU UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with HPLC-MS, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) has higher specificity and accuracy, but there are few reports on the application of this method at present, and no Relevant information

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An efficient and accurate method for detecting melatonin content in Paeoniae officinalis
  • An efficient and accurate method for detecting melatonin content in Paeoniae officinalis
  • An efficient and accurate method for detecting melatonin content in Paeoniae officinalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Instruments and reagents: Agilent6460 liquid mass spectrometer (Agilent, USA), Syncronis AQ-C18 chromatographic column (Thermo, USA), vacuum centrifugal concentrator (Eppendorf, Germany), KQ-200VDB ultrasonic cleaner (Kunshan, Jiangsu, ultrasonic Instrument Co., Ltd.), HC-2518 centrifuge (HKUST Innovation Co., Ltd.); analytical balance (Beijing Sartorius Scientific Instrument Co., Ltd.); microporous membrane (0.22 μm) (Shanghai AMP Experimental Technology Co., Ltd.).

[0043] Melatonin standard (purity ≥ 98%, Sigma Company, USA), methanol (HPLC grade, Tedia Reagent Company, USA), acetonitrile (HPLC grade, Tedia Reagent Company, USA), and deionized water were prepared by Millipore ultrapure water system.

[0044] 2. Preparation of the sample solution to be tested: the peony cultivar 'Hongfeng' planted in the peony germplasm resource nursery (32°30'N, 119°25'E) of the School of Horticulture and Plant Protection of Yangzhou University in the field was used as a test mate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for efficiently and accurately detecting the content of melatonin in peonies. The method comprises the steps of preparing a reference substance solution, preparing a test sample solution and detecting by high performance liquid chromatography-tandem mass spectrometry. The chromatographic peak obtained by detecting the melatonin in peony in the present invention can achieve baseline separation from the chromatographic peaks of other components, and is a symmetrical Gaussian peak with good peak shape, which can meet the qualitative and quantitative requirements; the melatonin in peony provided by the present invention The content detection method has the advantages of simple operation, high sensitivity, strong specificity, good stability, and accurate and reliable results.

Description

technical field [0001] The invention relates to a method for efficiently and accurately detecting the content of melatonin in peonies. Background technique [0002] Peony (Paeonia lactiflora Pall.) is a perennial herbaceous plant of Paeoniaceae, which has high ornamental and medicinal value. The medicinal use of peony is mainly to extract its root system, and its aerial parts have no other use except for ornamental purposes, especially the petals. After the flowering period every year, the petals fall all over the ground, which has virtually caused a huge amount of resources. waste. Melatonin is an indole neuroendocrine hormone that has attracted much attention in recent years. It has many important physiological functions for the human body, such as improving sleep quality, enhancing immunity, slowing down aging, anti-tumor, Antioxidant and regulate circadian rhythm etc. Previous studies have shown that after eating fruits and vegetables rich in melatonin, the level of m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陶俊赵大球刘定李志远陆春良
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap