Floating and shaking constant temperature anti-pollution cell thawing cup

Abstract

The invention discloses a constant-temperature type anti-pollution cell thawing cup with a floating oscillation function. The constant-temperature type anti-pollution cell thawing cup comprises a thawing cup body, a supporting cover, freezing tubes and lifting handles, wherein through holes for accommodating the freezing tubes are formed in the supporting cover, the supporting cover can be nested at the upper end of the thawing cup body in a matched manner, and a layer of floating bodies is composited at the bottom of the supporting cover and can be strong enough to float the supporting cover and all freezing tubes; a mounting hole is formed in the bottom of the thawing cup body, a stirring motor is mounted in the mounting hole in a sealed manner, and a rotating shaft of the stirring motor extends into the thawing cup body and equipped with stirring blades. Firstly, under the action of buoyancy, the freezing tubes are sufficiently contacted with a liquid to be in an optimal heat exchange state all along; the stirring motor is adopted to produce oscillation continuously; a counterweight plate is matched with the stirring motor, so that shaking amplitude and shaking frequency of each freezing tube can be improved, and a freezing liquid is better thawed.

Description

technical field [0001] The invention relates to the technical field of cell freezing tube thawing and resuscitating, in particular to a floating and oscillating constant temperature anti-pollution cell thawing cup. Background technique [0002] At present, the most effective way to preserve living cells is the liquid nitrogen ultra-low preservation method. In this method, organic solvents are used as protective agents, and they are preserved at ultra-low temperature (-196°C) in liquid nitrogen. The main principle is that the strain cells transition from normal temperature to low temperature, and before dropping to low temperature, the free water in the cell seeps out through the cell membrane, so as to prevent the cell from being damaged by the condensation of free water into ice crystals in the membrane. The key issues of using liquid nitrogen cryopreservation method are: 1. The choice of protective agent components and the concentration of each component; 2. The operati...

AI Technical Summary

Problems solved by technology

The key issues of using liquid nitrogen cryopreservation method are: 1. The choice of protective agent components and the concentration of each component; 2. Operation steps of cryopreservation freezing stage and thawing recovery stage

However, there are still problems in this solution: (1) The support mechanism is provided with outwardly turned support rods on both sides of the loading grate to solve the problem that the loading grate cannot be taken out after falling into the thawing cup

Therefore, in this patent document, the support rod constrains the loading grate so that the position of the loading grate on or below the liquid surface is uncertain, resulting in insufficient contact between the freezing tube and the liquid surface, and the thawing effect is different each time, and the effect is not good.

(2) Due to the slow natural heat transfer speed of the water temperature, when this patented technology is thawing in batches, all the freezing tubes are densely packed in the area below the liquid surface of the thawing cup, resulting in the rapid cooling of the upper water temperature in the thawing cup, and the lower water temperature cannot be thawed effectively. Thus delaying the thawing time, the ice crystals formed will scratch the cells

(3) During the thawing process, the patent document does not mention the means or revelation of thawing the frozen liquid by rapid shaking

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