Rapid propagation method of potted gerbera jamesonii bolus tissue culture

A tissue culture, rapid technology, applied in the field of agricultural science, can solve the problem of high cost, and achieve the effect of simple operation, high reproduction coefficient, and high survival rate of transplanting

Inactive Publication Date: 2017-05-17
太仓戈林农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention proposes a method for rapid propagation of potted gerbera through tissue culture, which solves the problem of relatively high cost of propagating potted gerbera by seeds in the prior art. The selection and di

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  • Rapid propagation method of potted gerbera jamesonii bolus tissue culture
  • Rapid propagation method of potted gerbera jamesonii bolus tissue culture
  • Rapid propagation method of potted gerbera jamesonii bolus tissue culture

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Embodiment 1

[0016] A kind of tissue culture rapid propagation method of potted gerbera, comprising the following steps: (1) selection and disinfection of explants: when the mother plant of potted gerbera, which survives and grows vigorously after being introduced, is placed in a ventilated place for one week, Take strong and complete flower buds (flower stalks are 2-3cm long), gently scrub them with detergent and rinse them under running tap water for 1 hour. Take the flower bud and place it under the ultra-clean table, wash it with 70% alcohol for 10 seconds, then rinse it with sterile water for 3 times (about 15 seconds each time), then add a drop of Tween 20 to 0.1% mercuric chloride, and shake for 5-8 minutes , and finally rinsed 7-10 times with sterile water. Cut the receptacle into small pieces with a side length of about 0.5 cm as explants. (2) Primary culture to induce adventitious buds: inoculate the explants obtained in step (1) into 1 auxin-free and 3 different auxin-added In ...

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Abstract

The invention discloses a rapid propagation method of potted gerbera jamesonii bolus tissue culture. The method comprises the following steps: shearing a gerbera jamesonii bolus receptacle into small blocks, sterilizing and placing in an MS culture medium containing 0.5mg/L of 6-BA and 0.1mg/L of NAA to induce, producing adventitious bud at an explant edge to acquire a sterile young plant, continuously placing blades of the sterile young plant in the culture medium as the explants, subculturing in the culture medium to obtain a robust plant, transferring the obtained robust plant into an MS rooting culture medium containing 0.3g/L of IBA to culture for 25-30 days to obtain a complete rooted plant, picking out an acquired rooted seedling from a culture bottle, cleaning and transplanting into a 72-cavity plate filled with matrix, gradually lowering relative air humidity to perform domestication and cultivation for 6 weeks, namely acquiring a transplanting stock. The young seedling obtained by use of the method disclosed by the invention is robust and high in survival rate, a great deal of high quality seedlings of the potted gerbera jamesonii bolus can be provided within short term, and the method has important effect on the scale promotion of the potted gerbera jamesonii bolus.

Description

technical field [0001] The invention relates to a rapid propagation method of potted gerbera through tissue culture, belonging to the field of agricultural science and technology. Background technique [0002] Gerbera jamesonii Bolus (Gerbera jamesonii Bolus), also known as gerbera and gerbera, is a perennial herb of the genus Gerbera in the family Asteraceae. Become one of the best-selling flowers in the world flower market. The cultivation of cut gerbera in my country is relatively common, and it occupies a large share in the fresh cut flower market. The potted gerbera is a newly developed potted flower variety in the world in recent years. The varieties of gerbera in the market are almost all cut flower varieties of tissue culture propagation, and there are few varieties specially used for potted flower cultivation. Compared with cut gerbera, potted gerbera or potted gerbera differs mainly in that the plant is shorter in plant type, the flower stem is thicker, the leave...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 卜崇兴樊晓亮
Owner 太仓戈林农业科技有限公司
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