Application of gastric cancer genes

一种基因、胃癌的技术，应用在重组DNA技术、微生物的测定/检验、DNA / RNA片段等方向，能够解决尚未有胃癌等问题，达到降低医疗成本、避免过度用药的效果

Active Publication Date: 2017-06-13
NANJING KDRB BIOTECH INC LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Genomic Health has also developed the Oncotype DX gene detection project for prostate cancer and colon cancer, but so far there is no similar detection for the prognosis of gastric cancer in the world

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] System verification using the TCGA public database of gastric cancer patients:

[0034] The prognostic scoring system was applied to 253 TCGA gastric cancer patients with survival data. Prognostic scores were used to predict the probability of survival for each individual patient. We divided the patients into two groups according to the prognostic score. If the prediction score ≤ -2, we defined the patient as having a good prognosis gene signature; if the prediction score > -2, we defined the patient as having a poor prognosis gene signature. Such as Figure 5 As shown, the survival time of the former was significantly longer than that of the latter. More than 50% of patients in the former were still alive after 100 months, while all patients in the latter died within 80 months.

[0035] In the literature, the expression difference method has been used to show the correlation of genes or multiple gene groups with the prognosis of gastric cancer. One question is whe...

Embodiment 2

[0037] Using the GSE15459 public database for gastric cancer patients to verify survival:

[0038] Using the same method, we verified the application value of the prognostic scoring system in the GSE15459 public database. Although the gastric cancer tissue gene expression value of this database is determined by Affymetrix chip technology, resulting in differences in the expression level baseline and scale, resulting in differences in the absolute value of the prediction score, but the scoring system of the present invention can still successfully predict gastric cancer prognosis ( Image 6 ).

Embodiment 3

[0040] To predict the prognosis of clinical gastric cancer patients:

[0041] Collect and extract RNA from tumor tissues of gastric cancer patients received clinically. Tumor tissues may include fresh biopsy tissues, postoperative tissues, fixed tissues and paraffin-embedded tissues. Then, the expression level of the 53 genes of the prognosis scoring system was quantitatively detected by using the kit developed by the present invention and corresponding instruments. The expression level of 53 genes is entered into the prognosis scoring formula that the present invention establishes:

[0042]

[0043] After calculating the predictive score of the patient, the doctor predicts the prognosis of the patient (see Example 1), such as the 5-year survival rate, according to the score. At present, we have established a model through retrospective research and successfully verified it in different databases. And started a prospective study to further improve the scoring system.

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Abstract

The invention discloses an application of a group of gastric cancer related genes. According to the invention, prediction scores are calculated to assess clinical prognosis of gastric cancers and related applications of the clinical prognosis of the gastric cancers based on a group of 53 prognostic related genes in the gastric cancers and a detection result of the expression level of the related genes in a clinical sample. The system can be used for helping gastric cancer patients in therapeutic choices of and prediction of responses to therapeutic intervention to judge whether the patients benefit from chemical and targeted therapies or not, so as to avoid excessive use of drugs and lower medical costs, and finally reach the purposes of precision or personalized medicine. According to the system and different detection technology platforms, corresponding kits for measuring expression of the 53 genes are designed and developed.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, and in particular relates to the application of a group of gastric cancer-related genes. Background technique [0002] Gastric cancer is a malignant tumor that starts from the epithelial cells of the gastric mucosa. Gastric cancer has always been one of the most common malignant tumors in the world. Its incidence is second only to lung cancer, breast cancer, colorectal cancer and prostate cancer, and ranks fifth in the incidence of cancer in the world. The incidence and mortality of gastric cancer have always ranked first. Although the overall incidence and mortality of gastric cancer in the world have declined in the past ten years, the absolute number of people suffering from gastric cancer is still on the rise, with nearly one million new cases every year. There are about 400,000 new cases in my country every year, accounting for 42% of the total number of cases in the world. Ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158C12Q2600/106
Inventor 杭渤王频李斌毛建华
Owner NANJING KDRB BIOTECH INC LTD
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