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Detection method for rapidly and definitely diagnosing feline leukemia and preparation of test strip thereof

A technology for preparing test strips and antibodies, which is applied in the field of medical testing, can solve problems such as poor accuracy, low sensitivity, and weak detection signals of colloidal gold, and achieve the effect of saving detection time

Inactive Publication Date: 2017-06-13
江苏雷森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection cost of enzyme-linked immunosorbent assay is relatively high, and there are many influencing factors in the detection process, which is prone to false positive results. For the detection of feline leukemia, the accuracy of enzyme-linked immunosorbent assay is not good
At the same time, the weak detection signal and low sensitivity of colloidal gold limit its application.

Method used

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  • Detection method for rapidly and definitely diagnosing feline leukemia and preparation of test strip thereof
  • Detection method for rapidly and definitely diagnosing feline leukemia and preparation of test strip thereof
  • Detection method for rapidly and definitely diagnosing feline leukemia and preparation of test strip thereof

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Effect test

Embodiment 1

[0036] A fluorescent stability verification of a fluorescent microsphere-labeled antibody for feline leukemia detection, comprising the following steps:

[0037] (1) Add a quantitative fluorescent latex microsphere solution to a buffer solution for dilution, and ultrasonicate for 1.5 min to obtain a fluorescent microsphere dispersion.

[0038] (2) Quickly drop the activator into the fluorescent latex microsphere dispersion in step (1), place it on a vortex shaker for 1 minute at a low speed, fix it on a vortex shaker, and shake it at room temperature for 30 minutes to activate.

[0039] (3) After the activation, the fluorescent latex microsphere dispersion in step (2) was centrifuged at 26° C. at 14000 rpm for 12 minutes, then the supernatant was removed, washed with the buffer solution in step (1), and then washed with the same method again. After conditioned centrifugation, discard the supernatant and reconstitute and resuspend with the buffer solution in step (4).

[0040]...

Embodiment 2

[0059] A preparation method for a test strip for rapid detection of feline leukemia virus, comprising the following steps:

[0060] (1) Add a quantitative fluorescent latex microsphere solution to a buffer solution for dilution, and ultrasonicate for 1.5 min to obtain a fluorescent microsphere dispersion.

[0061] (2) Quickly drop the activator into the fluorescent latex microsphere dispersion in step (1), place it on a vortex shaker for 1 minute at a low speed, fix it on a vortex shaker, and shake it at room temperature for 30 minutes to activate.

[0062] (3) After the activation, the fluorescent latex microsphere dispersion in step (2) was centrifuged at 26° C. at 14000 rpm for 12 minutes, then the supernatant was removed, washed with the buffer solution in step (1), and then washed with the same method again. After conditioned centrifugation, discard the supernatant and reconstitute and resuspend with the buffer solution in step (4).

[0063] (4) FeLv monoclonal antibody ...

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Abstract

The invention provides a detection method for rapidly and definitely diagnosing feline leukemia and a preparation method of a test strip thereof. Feline leukemia virus (abbreviated as FeLv) is used as a target to prepare the corresponding test strip by virtue of a chemical covalent coupling method, and the test strip is applied to the detection of the feline leukemia virus by utilizing a fluorescent labeled immnochromatography assay. The test strip for rapidly detecting the feline leukemia virus provided by the invention is high in sensitivity; a negative / positive result can be obtained, the content of the feline leukemia virus in a sample can also be quantitatively obtained, the test result is clearer and relatively high in stability, and the test strip can be stored for a long time at a room temperature.

Description

technical field [0001] The invention relates to a detection method for rapid diagnosis of feline leukemia and preparation of test strips thereof, belonging to the field of medical examination. Background technique [0002] Feline leukemia virus (FeLv) is a common cause of worldwide feline infection and is associated with high morbidity and mortality. Common symptoms of feline leukemia include malignant lymphoma, myelogenous leukemia, degenerative thymic atrophy and nonregenerative anemia, among which malignant lymphoma is the most serious for cats. Feline leukemia virus (FeLv) is characterized by causing lifelong infection in cats, with no obvious symptoms in the early stage, followed by persistent viremia, difficult to treat later, and eventually fatal results. Accurately diagnosing feline leukemia in the early stage of the disease and giving timely treatment is the best choice to cure feline leukemia. Sick cats with persistent infection have become the direct source of i...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/533
CPCG01N33/56983G01N33/533G01N2333/15
Inventor 秦雷朱丹丹张旭东陈芳沙森许席席彭雪婷秦允震卓雨晴谭影影
Owner 江苏雷森生物科技有限公司
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