Double haploid breeding method of porphyra haitanensis

Abstract

The invention relates to a double haploid breeding method of porphyra haitanensis. The method comprises the following steps of: selecting cross parents; promoting pure protonema with mature parents for diffuse conchospore culture to obtain parent thalli; selecting male and female thalli to hybridize; after culturing the male and female thalli, removing the male thalli, keeping the female thalli to culture singlely till the carpospores is released; culturing the single carpospores to be germinated to a synthesized protonema, and crushing the protonema for continuous culture; taking out part of the protonema to promote manurity, releasing the conchospore and filtering the same to culture to obtain F1 generation thalli, picking out the thalli, putting algae blocks in different colors in a glucose solution to be soaked for enzymolysis, and when single cells or multicellular aggregates are dissociated from the algae fragments, picking out single cells to culture; performing further cultivation till the culture liquid is restored to normal disinfecting seawater, wherein the double haploid is successfully induced; sucking out the pure protonema for amplified cultivation and storing the same as germplasm; and then taking out part of the protonema to promote maturity, promoting matured pure protonema to diffuse the conchospore, and collecting the conchospore to culture to obtain F2 generation pure thalli.

Description

technical field [0001] The invention relates to algae laver, in particular to a method for breeding algae double haploid (Double haploid). Background technique [0002] Pyropia haitanensis is one of the two main types of laver artificially cultivated in my country. It is native to the coast of Fujian Province and is a unique warm temperate species in my country. Since the success of artificial cultivation in the 1960s, the cultivation area of ​​Porphyra laver has continued to expand, and it has become one of the main targets of mariculture in southern my country. Its output accounts for more than 75% of the total production of laver in the country. economic benefits. With the development of the laver industry, the need for improved varieties is more urgent and diversified. The traditional breeding methods of laver include selective breeding, mutation breeding, hybrid breeding, etc., all of which require multiple generations of genetic purification to obtain stable traits. I...

AI Technical Summary

Problems solved by technology

Breeding using the method of inducing double haploids can quickly obtain homozygous individuals; in addition, because there is no dominant recessive effect between genes in double haploids, some traits determined by recessive genes can also be fully utilized , so double haploid induction is an effective way to accelerate the purification of germplasm materials and shorten the breeding period in breeding. It has been widely used in artificial breeding of plants, but it has not been reported in the breeding of macroalgae