Sperm washing liquid and preparation method thereof

Abstract

The invention relates to a sperm washing liquid and a preparation method thereof and belongs to the technical field of human assisted reproduction. The sperm washing liquid is prepared by dissolving heparin sodium dry powder or heparin sodium salt solution in a salt solution containing antibiotics and indicator. Heparin sodium is added into the sperm washing liquid, and by combination with a receptor on the surface of sperm, heparin can activate a signal transduction system coupled with the receptor, so a capacity function of sperm mitochondria and calcium ion internal flow are improved mildly and effectively, sperm activity is improved, and not only can the artificial insemination success rate be improved, but also safety of the assisted reproductive technology is strongly ensured.

Description

technical field

[0001] The invention relates to a sperm washing liquid and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique

[0002] Sperm washing technology is often used in assisted reproductive technology, the main purpose is to eliminate the factors that are not conducive to fertilization in the semen, so as to improve the success rate of IVF.

[0003] This technology is mainly based on the non-liquefaction of semen, the degree of antibody distribution, or infertility caused by other reasons for sperm, and it is used as an adjuvant treatment. The operation steps are: first, dilute the obtained sperm with washing solution as soon as possible to remove as many unfavorable components as possible, including seminal plasma, dead sperm, inflammatory cells, sperm antibodies, bacteria, mycoplasma, etc.; then, dilute the diluted Centrifuge the semen at a speed of 1000-2000 rpm for 10 minutes to precipitate the sp...

Examples

Embodiment Construction

[0027] The present invention will be further described below in combination with specific implementation methods.

[0028] The sperm washing solution of the present invention is prepared by dissolving heparin sodium dry powder or heparin sodium salt solution in a functionally feasible concentration (preferably 10-50 USP / ml) in a saline solution containing antibiotics and indicators. The antibiotic is gentamicin with a concentration of 5-11 μg / ml. The indicator is phenol red with a concentration of 9-10 μg / ml.

[0029] The saline solution is a compound culture solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it contains: Sodium 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, magnesium sulfate 0.18-0.22mmol / L, calcium chloride 1.92-2.12mmol / L, sodium bicarbonate 3.90-4.10mmol / L, HEPES or MOPS 19.99-22.00mmol / L, glucose 2.68-2.88mmol / L,...

technical field

[0001] The invention relates to a sperm washing liquid and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique

[0002] Sperm washing technology is often used in assisted reproductive technology, the main purpose is to eliminate the factors that are not conducive to fertilization in the semen, so as to improve the success rate of IVF.

[0003] This technology is mainly based on the non-liquefaction of semen, the degree of antibody distribution, or infertility caused by other reasons for sperm, and it is used as an adjuvant treatment. The operation steps are: first, dilute the obtained sperm with washing solution as soon as possible to remove as many unfavorable components as possible, including seminal plasma, dead sperm, inflammatory cells, sperm antibodies, bacteria, mycoplasma, etc.; then, dilute the diluted Centrifuge the semen at a speed of 1000-2000 rpm for 10 minutes to precipitate the sp...

Examples

Embodiment Construction

[0027] The present invention will be further described below in combination with specific implementation methods.

[0028] The sperm washing solution of the present invention is prepared by dissolving heparin sodium dry powder or heparin sodium salt solution in a functionally feasible concentration (preferably 10-50 USP / ml) in a saline solution containing antibiotics and indicators. The antibiotic is gentamicin with a concentration of 5-11 μg / ml. The indicator is phenol red with a concentration of 9-10 μg / ml.

[0029] The saline solution is a compound culture solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it contains: Sodium 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, magnesium sulfate 0.18-0.22mmol / L, calcium chloride 1.92-2.12mmol / L, sodium bicarbonate 3.90-4.10mmol / L, HEPES or MOPS 19.99-22.00mmol / L, glucose 2.68-2.88mmol / L,...