American cockroach water-soluble protein as well as extraction process and application thereof

A technology of water-soluble protein and Periplaneta americana, which is applied in the field of water-soluble protein of Periplaneta americana and its extraction process and application, can solve problems that are rarely involved in the research and application of protein peptides, and achieve increased concentration, high quality, and high efficiency The effect of the extraction process

Inactive Publication Date: 2017-08-29
SICHUAN GOODDOCTOR PANXI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the relevant research and development applications are mainly focused on smal

Method used

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Experimental program
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Embodiment 1

[0023] After the Periplaneta americana body was dried, it was pulverized into powder by a pulverizer; the powder and anhydrous ether were added into anhydrous ether at a solid-to-liquid ratio of 1:10 (W:V), and degreased at 50°C for 1 hour, and then Suction filtration, discard oil filtrate I, solid part I, then add anhydrous ether at a solid-to-liquid ratio of 1:10, perform second degreasing under the same environment and at the same time, after suction filtration, discard oil filtrate II, and keep solid part II. Add Tris-HCl buffer solution with pH=9 to the solid part II at a solid-to-liquid ratio of 1:10 (W:V), treat at 35°C for 6 hours, centrifuge at 4000 rpm for 10 minutes, save the supernatant I, and precipitate I Add Tris-HCl buffer solution with pH=9 again at a solid-to-liquid ratio of 1:10 (W:V), treat at 35°C for 6 hours, then centrifuge at 4000 rpm for 10 minutes, discard the precipitate II, and keep the supernatant II. Mix the supernatant I and the supernatant II, f...

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Abstract

The invention provides an American cockroach water-soluble protein as well as an extraction process and application thereof. According to the technical scheme, the process is realized through the following steps: a, degreasing; b, performing crude protein extraction; c, dialyzing; and d, drying, thereby obtaining the product. The protein concentration of the American cockroach water-soluble protein obtained by the extraction process disclosed by the invention reaches 8121.531 [mu]g/ml, the concentration of an American cockroach extracting solution obtained through a salt extraction method is 4913.55 [mu]g/ml, and the advantages of the extraction process disclosed by the invention can be obviously seen through comparison. The American cockroach water-soluble protein disclosed by the invention can be applied to foods or medicines or feed additives.

Description

technical field [0001] The invention belongs to the field of insect protein preparation, in particular to a water-soluble protein of Periplaneta americana and its extraction process and application. Background technique [0002] Under the trend of rapid population growth in the world today, the food crisis and the accompanying problem of human protein deficiency are becoming more and more serious. Finding new food sources and developing edible protein sources are great challenges we face. Insect protein is currently the most promising new protein resource. It has the following characteristics: rich in nutrient components, high in nutritional value, wide in source, fast in reproductive cycle, high in reproductive index, large in output, and easy to process. With the intensification of competition and the increase of demand for natural green protein, comprehensive and ingenious utilization of insects to explore their protein resources has gradually attracted widespread atten...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/34
CPCC07K14/43563
Inventor 耿福能鲍锦库岳碧松沈咏梅刘彬
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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