Method for specificity shRNA screening and verification on lung cancer cell inhibition of targeted Ang-2 gene of shRNA

Abstract

The invention discloses a method for specificity shRNA screening and verification on lung cancer cell inhibition of a targeted Ang-2 gene of shRNA. Specificity shRNA screening and verification on lung cancer cell inhibition of the targeted Ang-2 gene of the shRNA are completed through the steps of cell recovery, cell culture, cell transfection, Ang-2 expression detection through real-time fluorescent quantitative PCR before and after interference, expression analysis of protein in cells before and after interference, CCK-8 method based detection of lung cancer cell proliferation capability before and after Ang-2 gene interference and detection of cell invasion and migration capability change before and after interference. Screening of Ang-2-shRNA1 effective plasmid transfected lung cancer cells having specificity to Ang-2 gene transcription interference is achieved, meanwhile it can be verified that specificity shRNA targeted interference Ang-2 can effectively inhibit proliferation, invasion, migration and other biological capabilities of the cancer cells, a new treatment direction is provided for lung cancer treatment, the Ang-2 is inhibited, and the limitation of a single anti-VEGFR treating method is made up.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a specific shRNA screening method and a verification method for targeting Ang-2 gene to inhibit lung cancer cells. Background technique [0002] Lung cancer is still one of the most common human malignant tumors and the main cause of cancer-related death in the world today. The 5-year survival rate of lung cancer is low, the recurrence rate is high after surgery, and the prognosis is poor. Even after surgery, the 10-year survival rate of patients with stage I lung cancer Up to 90%. The treatment method is surgery-based comprehensive treatment supplemented by radiotherapy and chemotherapy. However, due to reasons such as metastasis and drug resistance, the curative effect of various chemotherapy drugs is reduced or even ineffective. Although there are a variety of targeted drugs that can prolong the survival time of lung cancer patients, they still cannot reduce the mortality ...

AI Technical Summary

Problems solved by technology

The treatment method is mainly surgery, supplemented by radiotherapy and chemotherapy. However, due to reasons such as metastasis and drug resistance, the efficacy of various chemotherapy drugs is reduced or even ineffective.

Although there are a variety of targeted drugs that can prolong the survival time of lung cancer patients, they still cannot reduce lung cancer mortality. New molecular markers and molecular targets still need to be explored as new treatment strategies for lung cancer

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