Method for establishing animal in-vitro tracheal model for evaluating inflammatory response induced by tobacco mainstream smoke and evaluation method

Abstract

The invention relates to a method for establishing an animal in-vitro tracheal model for evaluating inflammatory response induced by tobacco mainstream smoke and an evaluation method, and belongs to the technical field of cigarette hazard assessment. The method for establishing the animal in-vitro tracheal model comprises the following steps: 1) separating a tracheal ring; 2) culturing the tracheal ring obtained in the step 1) in vitro; and 3) exposing the tracheal ring cultured in the step 2) to a flue gas condensate for 1 to 24 hours. The evaluation method of the inflammatory response induced by the tobacco mainstream smoke comprises selection of evaluation indexes and division of evaluation criteria; and evaluation of the degree of the inflammatory response. The method has the followingadvantages: 1) the detection method of the tracheal inflammation after smoke exposure has high efficiency; 2) the detection method of the tracheal inflammation after smoke exposure has good repeatability and system stability and high accuracy; and 3) the use range is wide. The method can be applied to in vitro exposure models of cigarette mainstream smoke, sidestream smoke, ambient flue gas and other smoke aerosols.

Description

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AI Technical Summary

Benefits of technology

This technology allows for effective monitoring during smoking or burning cigarettes by measuring changes caused by various factors like air pollution (smoke), nicotine levels, etc., which are associated with lung damage from these substances. It also provides reliable results even when exposed repeatedly over time without losing accuracy due to degraded performance.

Problems solved by technology

The technical problem addressed in this patented paper relating to evaluating the effects of reducing smoker induced lung injury during experimental testing with rodent models that only allow for specific responses from these mechanisms without affecting others like those involved in normal breathing behavior. This requires developing new assays capable of predicting how well gas permeability changes occur when exposed to harmful agents while maintaining their ability to replicate complex interactions within the body under physically controlled conditions.

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