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Environmental protection repairing agent and method for heavy metal pollution in soil

A remediation method and heavy metal technology, applied in the restoration of polluted soil, fertilizer mixture, fermentation, etc., can solve the problems of pollutants and heavy metals cannot be degraded by microorganisms, and the environment is long-term, so as to improve soil fertility and improve the microbial environment of plant roots. , the effect of promoting root growth

Active Publication Date: 2018-01-23
米涛(上海)环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. Heavy metals cannot be degraded by microorganisms and are long-term and potential pollutants in the environment;

Method used

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  • Environmental protection repairing agent and method for heavy metal pollution in soil
  • Environmental protection repairing agent and method for heavy metal pollution in soil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of rhamnolipid biosurfactant

[0032] Pseudomonas aeruginosa CCTCC AB 2013184 was added to the fermentation medium at an inoculum size of 3% (V / V), and cultured on a shaker at 37°C at a speed of 280 rpm for 90 hours to obtain a fermentation broth;

[0033] The composition of the fermentation medium is olive oil 60g / L, sodium nitrate 8g / L, sodium chloride 1.0g / L, potassium chloride 1.0g / L, calcium chloride 0.1g / L, KH 2 PO 4 12H 2 O 3.5g / L, magnesium sulfate 0.25g / L, FeSO 4 ·7H 2 O 0.001g / L, the balance is water, pH6.6.

[0034] The fermentation broth was incubated at 120°C for 30 minutes, then cooled to 40°C, centrifuged at 10,000 rpm for 5 minutes to remove bacterial debris, and the supernatant was extracted with an equal volume of ethyl acetate, and then used a rotary evaporator Distill under reduced pressure to control the rhamnolipid content to 10g / L to obtain the bio-environmental friendly surfactant of the present invention.

Embodiment 2

[0035] Embodiment 2: the preparation of rhamnolipid biosurfactant

[0036] Pseudomonas aeruginosa CCTCC AB 2013184 was added to the fermentation medium at an inoculum size of 3% (V / V), and cultured on a shaker at 37°C at a speed of 280 rpm for 90 hours to obtain a fermentation broth;

[0037] The composition of the fermentation medium is 60g / L of safflower seed oil, 8g / L of sodium nitrate, 1.0g / L of sodium chloride, 1.0g / L of potassium chloride, 0.1g / L of calcium chloride, KH 2 PO 4 12H 2 O 3.5g / L, magnesium sulfate 0.25g / L, FeSO 4 ·7H 2 O 0.001g / L, the balance is water, pH6.6.

[0038] The fermentation broth was incubated at 120°C for 30 minutes, then cooled to 40°C, centrifuged at 10,000 rpm for 5 minutes to remove bacterial debris, and the supernatant was extracted with an equal volume of ethyl acetate, and then used a rotary evaporator Distill under reduced pressure to control the rhamnolipid content to 10g / L to obtain the bio-environmental friendly surfactant of the ...

Embodiment 3

[0039] Embodiment 3: the preparation of rhamnolipid biosurfactant

[0040] Pseudomonas aeruginosa CCTCC AB 2013184 was added to the fermentation medium at an inoculum size of 3% (V / V), and cultured on a shaker at 37°C at a speed of 280 rpm for 90 hours to obtain a fermentation broth;

[0041] The composition of the fermentation medium is olive oil 20g / L, safflower seed oil 40g / L, sodium nitrate 8g / L, sodium chloride 1.0g / L, potassium chloride 1.0g / L, calcium chloride 0.1g / L 、KH 2 PO 4 12H 2 O 3.5g / L, magnesium sulfate 0.25g / L, FeSO 4 ·7H 2 O0.001g / L, the balance is water, pH6.6.

[0042] The fermentation broth was incubated at 120°C for 30 minutes, then cooled to 40°C, centrifuged at 10,000 rpm for 5 minutes to remove bacterial debris, and the supernatant was extracted with an equal volume of ethyl acetate, and then used a rotary evaporator Distill under reduced pressure to control the rhamnolipid content to 10g / L to obtain the bio-environmental friendly surfactant of th...

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Abstract

The invention discloses an environmental protection repairing agent and method for heavy metal pollution in soil. The environmental protection repairing agent is made from, by weight, 10-30 parts of potassium fulvate, 10-30 parts of monopotassium phosphate, 10-20 parts of L-malic acid, 20-40 parts of rhamnolipid bio-surfactant, 1-3 parts of Sodium 2,3-dimercapto-1-propanesulfonate, and 2-8 parts of sodium carboxymethyl cellulose. The environmental protection repairing agent may be complexed with heavy metal ions in soil, thereby reducing soil interfacial tension, shielding activity of heavy metal ions, reducing toxicity of the heavy metal ions and decreasing the quantity of heavy metal ions absorbed by plants; in addition, the environmental protection repairing agent can provide phosphorusand potassium nutrition for soil and plants, and balance and improve soil nutritional condition, thereby improving soil fertility; the environmental protection repairing agent can also effectively improve plant root microbial environment, thereby improving metabolism of microbes, promoting root growth and improving crop quality.

Description

technical field [0001] The invention relates to an environment-friendly restoration agent for soil heavy metal pollution and an environment-friendly restoration method for soil heavy metal. Background technique [0002] Heavy metal pollution of the soil refers to human activities, the content of trace metal elements in the soil exceeds the background value, and the excessive content caused by excessive deposition is collectively referred to as soil heavy metal pollution. [0003] Heavy metals refer to metals with a specific gravity equal to or greater than 5.0, such as Fe, Mn, Zn, Cd, Hg, Ni, Co, etc.; As is a metalloid, but due to its chemical properties and environmental behavior are similar to heavy metals, Therefore, when discussing heavy metals, arsenic is often included, and some directly include it in the scope of heavy metals. Due to the high content of iron and manganese in soil, it is generally considered that they are not soil pollution elements, but under strong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04B09C1/08C12P7/62C05G3/80
Inventor 徐云丽
Owner 米涛(上海)环保科技有限公司
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