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A culture medium and detection method for detecting bifidobacteria

A bifidobacteria and culture medium technology, applied in the field of microbial detection, can solve the problems of poor bifidobacteria detection specificity, inability to accurately detect bifidobacteria, and difficulty in reading results, and achieve good inhibitory effect, easy interpretation, and specificity. good effect

Active Publication Date: 2020-10-30
BIOSTIME GUANGZHOU HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has good specificity for the detection of bifidobacteria in the bifidobacterium and lactobacillus combination nutrition, but the specificity for the detection of bifidobacteria in the lactic acid bacteria combination after a period of time after the number of viable bacteria has decreased is not good, and there will still be Both Lactobacillus and Bifidobacteria are present, Bifidobacteria cannot be accurately detected
Moreover, the colony growth of this detection method is particularly small, it is difficult to read accurately, and it is difficult to read the result

Method used

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  • A culture medium and detection method for detecting bifidobacteria
  • A culture medium and detection method for detecting bifidobacteria
  • A culture medium and detection method for detecting bifidobacteria

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1 Detection method of the present invention

[0041] 1. Equipment and materials

[0042] In addition to routine sterilization and culture equipment in the microbiology laboratory, other equipment and materials are as follows:

[0043] 1.1 Constant temperature incubator: 36℃±1℃;

[0044] 1.2 Homogenizer and aseptic homogenizing bag, homogenizing cup or sterilized mortar;

[0045] 1.3 Refrigerator: 2℃~5℃;

[0046] 1.4 Balance: Sensitivity 0.1g;

[0047] 1.5 Sterile test tube: 18mm×180mm, 15mm×100mm;

[0048] 1.6 Sterile pipette: 1mL (with 0.01mL scale), 10mL (with 0.1mL scale)

[0049] 1.7 Spiral inoculator or L-shaped glass smear rod

[0050] 1.8 Anaerobic incubator box or anaerobic tank and supporting consumables

[0051] 1.9 Vortex shaker

[0052] 2. Media and reagents

[0053] 2.1 Soy peptone

[0054] 2.2 MRS medium

[0055] 2.3 Lithium chloride: analytically pure

[0056] 2.4 Sodium propionate: chemically pure

[0057] 2.5 Raffinose: biochemic...

Embodiment 2

[0081] Three batches of Bifidobacterium and Lactobacillus combination products were selected, and each batch of products was repeated twice. Simultaneously use the improved MRS medium of mupirocin lithium salt specified in GB4789.35-2016 plus cysteine ​​hydrochloride (MRS+M+L) and the improved Columbia agar medium (RAF) described in Example 1 For detection, physiological saline and phosphate buffered saline were respectively selected as diluents. The results are shown in Table 1.

[0082] Table 1 detects the result of bifidobacterium

[0083]

[0084] Note: TNTC means too many to count; X means there are many small transparent colonies in the background, too many to count

[0085] The results in Table 1 show that in the enumeration level of bifidobacteria (10 -7 ), there are many small transparent colonies on the MRS+M+L plate, with a diameter of figure 2 ), and innumerable (TNTC), this method cannot inhibit the Lactobacillus helveticus in the product, and cannot accura...

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Abstract

The invention belongs to the technical field of microorganism detection, and discloses a culture medium for detecting bifidobacterium and a detection method. The culture medium for detecting the bifidobacterium comprises a base culture medium, lactose and cysteine hydrochloride, wherein the base culture medium is prepared from a Columbia blood agar base culture medium, raffinose, lithium chloride,sodium propionate and water, and the pH (potential of hydrogen) value is 5.1. The culture medium has the advantages that besides the Columbia blood agar base culture medium, the culture medium also contains the lactose, the raffinose and the cysteine hydrochloride, and the lactose, the raffinose and the cysteine hydrochloride are used as nutritional agents to promote the growth of the bifidobacterium; when the total number of bifidobacterium is detected by the culture medium, the large colony of the bifidobacterium grows, the judging is easy, and the specificity is good; the good inhibiting function on lactobacillus helveticus is realized, and the content of bifidobacterium in the bifidobacterium and lactobacillus combined nutritional product can be accurately detected.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a culture medium and a detection method for detecting bifidobacteria, in particular to a culture medium and a detection method for detecting bifidobacteria in a combination of bifidobacteria and lactobacilli. Background technique [0002] Fermented food is rich in lactic acid bacteria, which can ferment sugar to produce a large amount of lactic acid, which has a positive impact on the digestion and immune-related physiological functions of the human body. Common food lactic acid bacteria include several genera such as Lactobacillus, Bifidobacterium and Streptococcus thermophilus, and they are often mixed as needed to prepare functional food or nutritional products. However, there is no very accurate and specific method for the detection of this combination of lactic acid bacteria, especially for the detection of bifidobacteria. For the detection of La...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12Q1/06C12R1/01
CPCC12Q1/045C12Q1/06
Inventor 罗星晓付日留杨云黄惠英
Owner BIOSTIME GUANGZHOU HEALTH PROD
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