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34results about How to "Efficient localization" patented technology

Assay for localized detection of analytes

InactiveUS20110223585A1Efficient localizationSimple and convenient visualizationMicrobiological testing/measurementAssayAnalyte
The present invention relates to a method for detecting an analyte in a sample, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, wherein said probes each comprise an analyte-binding moiety and can simultaneously bind to the analyte, and wherein (i) said first proximity probe comprises a nucleic acid moiety attached at one end to the analyte-binding moiety, wherein a circular or circularizable oligonucleotide is hybridized to said nucleic acid moiety before, during or after said contacting step; and (ii) said second proximity probe comprises an enzyme moiety, attached to the analyte-binding moiety, capable of directly or indirectly enabling rolling circle amplification (RCA) of the circular or, when it is circularized, of the circularizable oligonucleotide hybridized to the nucleic acid moiety of the first proximity probe, wherein said RCA is primed by said nucleic acid moiety of said first proximity probe; (b) if necessary, circularizing said oligonucleotide, to produce a circularized template for RCA; (c) subjecting said circular or circularized template to RCA, wherein if the enzyme moiety of the second proximity probe in step (a)(ii) is a DNA polymerase, this step does not utilize a free DNA polymerase; and (d) detecting a product of said RCA.
Owner:OLINK AB

Safe distributed database interaction method suitable for mobile positioning system

The invention discloses a secure distributed database interaction method suitable for a mobile positioning system. The secure distributed database interaction method comprises seven modules, wherein arest data transceiving and attribution region division module is used for receiving client data and determining an attribution through an improved trie tree algorithm; a read-write separation local database module designs a statistical-based read-write separation algorithm to carry out read-write separation and temporary table establishment; a local key agreement module is used for designing a key agreement protocol on the basis of the pre-shared key and generating a session key; an attribution secret key negotiation module interacts with the local secret key negotiation module to generate asession secret key each time; a local data encryption and decryption and integrity protection module is responsible for encryption and decryption of local interaction data through an improved SM4 encryption algorithm; an attribution data encryption and decryption and integrity protection module is used for realizing encryption and decryption of attribution interaction data by designing and improving an SM4 encryption algorithm; and a read-write separation attribution database module is used for realizing read-write of the attribution database based on a statistical read-write separation algorithm.
Owner:SOUTHEAST UNIV
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