The invention provides biological toothpaste for resisting helicobacter pylori, and relates to the field of medical application. The biological toothpaste comprises the following substances in parts by weight: 25 parts of sorbitol, 1.5 parts of sodium carboxymethyl cellulose, 2.5 parts of sodium dodecyl sulfate, 13 parts of polyethylene glycol, 4 parts of calcium hydrogen phosphate, 20 parts of silicon dioxide, 0.3 part of xylitol, 1 part of broccoli essence, 0.5 part of eugenol, 0.5 part of tea tree essential oil, 0.3 part of lactoferrin, 1 part of probiotics and the balance of deionized water. In the biological toothpaste provided by the invention, the broccoli essence, lactoferrin and eugenol play a role in killing helicobacter pylori and cariogenic bacteria, the tea tree essential oilcan remove dental plaques and freshen breath, the xylitol can prevent dental caries, and the probiotics can balance oral floras. The raw materials are organically combined to prepare the toothpaste, which can play a role in eliminating oral helicobacter pylori, preventing dental caries and balancing oral floras, thereby realizing the purpose of the invention. The biological toothpaste provided bythe invention is simple to manufacture, is used as a daily necessity, is convenient and quick to use, economical and applicable, and good for long-term use, prevents and treats helicobacter pylori andcariogenic bacteria in an oral cavity, and keeps the oral cavity healthy.