Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof
A technology of Lactobacillus plantarum and Helicobacter pylori, which is applied in the field of microbiology, can solve the problem that there is no effective method for screening Lactobacillus against Helicobacter pylori, and achieve the effect of preventing or alleviating infection
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Embodiment 1
[0063] Example 1 : Screening of Lactobacilli that antagonize H.pylori SS1 using principal component analysis
[0064] 16 strains of Lactobacillus were screened from 238 strains of Lactobacillus. They grew well under low pH conditions and could tolerate acidic artificial gastric juice. The acid resistance test of these 16 strains of Lactobacillus was studied. Through acid resistance test, growth inhibition test of Helicobacter pylori (taking H.pylori SS1 as an example), urease activity detection, self-agglutination and hydrophobicity of lactobacillus and co-aggregation test of lactobacillus and H.pylori SS1, using The ability of these lactobacilli to antagonize H.pylori SS1 was screened by principal component analysis.
[0065] These experimental results show that the factor loading plot ( figure 1 ) is mainly divided into 4 components. The first component accounted for 28.87%, and the bacteriostasis was the main contribution; the second component accounted for 14.17%, and ...
Embodiment 2
[0066] Example 2 : Analysis of the antagonistic ability of Lactobacillus plantarum CN2018 against other Helicobacter pylori strains
[0067] Columbia culture medium (Oxiod, UK) was poured into plates (15 mL per plate, the plate was a standard plate of 9mm), and H. 1×10 8 CFU / plate), put it into 4 Oxford cups immediately, and add 100μL Lactobacillus fermentation liquid to the Oxford cup, fix it under microaerophilic conditions for 1h, put it into a three-gas incubator for 48-72h, and measure the antibacterial ring size. Using MRS as a negative control, the experiment was repeated three times, and the test results are listed in Table 1.
[0068] Table 1 Analysis of inhibition of growth of other Helicobacter pylori
[0069]
[0070] ZOI is the diameter of the zone of inhibition; SEM is the standard deviation;
[0071] The test results in Table 1 show that Lactobacillus plantarum CN2018 of the present invention has an inhibitory effect on these strains of Helicobacter pyl...
Embodiment 3
[0072] Example 3 : Analysis of organic acid content in Lactobacillus fermentation supernatant
[0073] The Lactobacillus plantarum CN2018 of the present invention is activated for two generations with MRS medium, and then cultured for 18 hours. The obtained fermentation broth was centrifuged at 4°C and 5000r / min for 5min, the obtained supernatant was filtered using a membrane with a pore size of 0.22μm, and the filtrate was diluted 10 times with sterile water, and then the high performance liquid chromatography was used under the following conditions: Next, detect its lactic acid content and other organic acid content.
[0074] Chromatographic conditions: instrument: Agilent 1100; chromatographic column: Ecosil, C18, 46×250mm, 50cm; mobile phase: methanol / H 3 PO 4 / water=5 / 0.05 / 95; flow rate: 1 mL / min; column temperature: 30°C; detection: UV 210nm; injection volume: 5 μL.
[0075] The test results are listed in Table 2.
[0076] Table 2: Analysis of organic acid content ...
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