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Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof

A technology of Lactobacillus plantarum and Helicobacter pylori, which is applied in the field of microbiology, can solve the problem that there is no effective method for screening Lactobacillus against Helicobacter pylori, and achieve the effect of preventing or alleviating infection

Active Publication Date: 2011-09-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these documents do not have an effective method for screening Lactobacillus against Helicobacter pylori

Method used

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  • Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof
  • Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof
  • Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 : Screening of Lactobacilli that antagonize H.pylori SS1 using principal component analysis

[0064] 16 strains of Lactobacillus were screened from 238 strains of Lactobacillus. They grew well under low pH conditions and could tolerate acidic artificial gastric juice. The acid resistance test of these 16 strains of Lactobacillus was studied. Through acid resistance test, growth inhibition test of Helicobacter pylori (taking H.pylori SS1 as an example), urease activity detection, self-agglutination and hydrophobicity of lactobacillus and co-aggregation test of lactobacillus and H.pylori SS1, using The ability of these lactobacilli to antagonize H.pylori SS1 was screened by principal component analysis.

[0065] These experimental results show that the factor loading plot ( figure 1 ) is mainly divided into 4 components. The first component accounted for 28.87%, and the bacteriostasis was the main contribution; the second component accounted for 14.17%, and ...

Embodiment 2

[0066] Example 2 : Analysis of the antagonistic ability of Lactobacillus plantarum CN2018 against other Helicobacter pylori strains

[0067] Columbia culture medium (Oxiod, UK) was poured into plates (15 mL per plate, the plate was a standard plate of 9mm), and H. 1×10 8 CFU / plate), put it into 4 Oxford cups immediately, and add 100μL Lactobacillus fermentation liquid to the Oxford cup, fix it under microaerophilic conditions for 1h, put it into a three-gas incubator for 48-72h, and measure the antibacterial ring size. Using MRS as a negative control, the experiment was repeated three times, and the test results are listed in Table 1.

[0068] Table 1 Analysis of inhibition of growth of other Helicobacter pylori

[0069]

[0070] ZOI is the diameter of the zone of inhibition; SEM is the standard deviation;

[0071] The test results in Table 1 show that Lactobacillus plantarum CN2018 of the present invention has an inhibitory effect on these strains of Helicobacter pyl...

Embodiment 3

[0072] Example 3 : Analysis of organic acid content in Lactobacillus fermentation supernatant

[0073] The Lactobacillus plantarum CN2018 of the present invention is activated for two generations with MRS medium, and then cultured for 18 hours. The obtained fermentation broth was centrifuged at 4°C and 5000r / min for 5min, the obtained supernatant was filtered using a membrane with a pore size of 0.22μm, and the filtrate was diluted 10 times with sterile water, and then the high performance liquid chromatography was used under the following conditions: Next, detect its lactic acid content and other organic acid content.

[0074] Chromatographic conditions: instrument: Agilent 1100; chromatographic column: Ecosil, C18, 46×250mm, 50cm; mobile phase: methanol / H 3 PO 4 / water=5 / 0.05 / 95; flow rate: 1 mL / min; column temperature: 30°C; detection: UV 210nm; injection volume: 5 μL.

[0075] The test results are listed in Table 2.

[0076] Table 2: Analysis of organic acid content ...

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Abstract

The invention relates to lactobacillus CN2018, CGMCC No. 4286 and application thereof. The lactobacillus CN2018 has acid resistance, has an effect of suppressing growth of helicobacter pylori (H. pylori SS1 for instance) and urease activity, has a stronger effect of suppressing the adherence of helicobacter pylori to human gastric epithelial cells SGC7901, and has an effect of preventing or relieving infection degree of mice infection of the helicobacter pylori.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a Lactobacillus plantarum capable of intervening Helicobacter pylori infection, and also relates to the use of the Lactobacillus plantarum. 【Background technique】 [0002] As the main pathogenic factor of chronic gastritis, peptic ulcer and even gastric cancer, Helicobacter pylori (H. pylori) has been plagued the whole world. As the original bacterium in the stomach, Helicobacter pylori can survive and colonize under strong acidic conditions. It has two main characteristics. The first characteristic is that Helicobacter pylori can produce a large amount of urease during the metabolic process, while Urease can decompose urea to produce ammonia. Therefore, an "ammonia cloud" microenvironment is formed around Helicobacter pylori, which can increase the pH and protect it from strong acid erosion. The second feature is that Helicobacter py...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04A23C9/12A23C19/032A23L1/20A23L1/212A23K1/16A23L1/29C12R1/25A23K10/16A23L11/50A23L19/00A23L33/00A61K35/747
Inventor 陈卫田丰伟陈晓华赵建新张灏刘小鸣张秋香
Owner JIANGNAN UNIV
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