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A kind of compound lactic acid bacteria microecological preparation and its preparation method and application

A micro-ecological preparation and compound lactic acid bacteria technology, applied in the biological field, can solve the problems such as the inability to achieve multi-angle, all-round and multi-functional regulation of the intestinal flora structure of pet cats, poor systemicity of a single strain, etc., and achieve optimal mental state and health. The effect of fur condition, cat litter odor reduction, good advancement and practicality

Active Publication Date: 2021-07-09
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, this application proposes a compound lactic acid bacteria microecological preparation and its preparation method and application to solve the problem of poor systemicity of a single strain and the inability to realize multi-angle, all-round and multi-functional regulation of the intestinal flora structure of pet cats. question

Method used

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  • A kind of compound lactic acid bacteria microecological preparation and its preparation method and application
  • A kind of compound lactic acid bacteria microecological preparation and its preparation method and application
  • A kind of compound lactic acid bacteria microecological preparation and its preparation method and application

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Embodiment 1

[0046] Preparation of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, Bifidobacterium animalis V9 and preparation of compound lactic acid bacteria microecological preparation

[0047] In step 1, Lactobacillus plantarum LP6, Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium animalis are respectively subjected to primary fermentation to obtain corresponding primary seed culture solutions.

[0048] The fermentation conditions of the primary fermentation include: Lactobacillus plantarum LP6, Lactobacillus casei, Lactobacillus plantarum and animal Bifidobacterium respectively at the inoculation amount of 2%-10% (v / v), temperature 33-37°C, rotation speed 50-100r Cultivate for 18-24 hours under the condition of / m; preferably, cultivate for 20-22 hours under the conditions of inoculum size 5%-8% (v / v), temperature 35-36°C, and rotational speed 60-80r / m.

[0049] The effective number of viable bacteria of Lactobacillus plantarum LP6 ...

Embodiment 2

[0101] The preparation of embodiment 2 compound lactic acid bacteria probiotics

[0102] By the same method as in Example 1, these four strains of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V9 were respectively carried out to primary, secondary seed culture, and tertiary fermentation culture. Centrifuge separately to obtain the cells of these four strains of bacteria, mix the cells with the protective agent solution according to the mass ratio of 1:5, 1:8, 1:5, and 1:10 to obtain a bacterial suspension, and the bacterial suspension The solution was freeze-dried to obtain bacterial agents respectively; said Lactobacillus plantarum LP6 bacterial agent, Lactobacillus casei zhang bacterial agent, Lactobacillus plantarum P-8 bacterial agent, animal Bifidobacterium V9 bacterial agent in a ratio of 1:1:1 :2 to mix, and add the dilution carrier to compound, to obtain the compound lactic acid bacteria microecologica...

Embodiment 3

[0103] The preparation of embodiment 3 compound lactic acid bacteria probiotics

[0104] By the same method as in Example 1, these four strains of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V9 were respectively carried out to primary, secondary seed culture, and tertiary fermentation culture. Centrifuge separately to obtain the cells of these four strains of bacteria, mix the cells with the protective agent solution according to the mass ratio of 1:7, 1:10, 1:5, and 1:8 to obtain a bacterial suspension, and the bacterial suspension The solution was freeze-dried to obtain bacterial agents respectively; said Lactobacillus plantarum LP6 bacterial agent, Lactobacillus casei zhang bacterial agent, Lactobacillus plantarum P-8 bacterial agent, animal Bifidobacterium V9 bacterial agent in a ratio of 1:1:1 : 7 for mixing, and adding the dilution carrier to compound, to obtain the compound lactic acid bacteria microe...

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Abstract

The present application provides a compound lactic acid bacteria micro-ecological preparation and its preparation method and application. The Mycobacterium V9 bacterial agent and the diluted carrier are mixed and compounded, and the number of viable bacteria of the four bacterial agents is ≥2×10 11 CFU / g. The content of live bacteria used is 1×10 9 The CFU / g compound lactic acid bacteria microecological preparation was tested on 30 pet cats of different breeds for 30 days, which can improve the health level of pet cats. Reduce the degree of hair loss, reduce the ammonia concentration in the feces, reduce the smell of cat litter, significantly increase the feed intake and growth rate, and significantly increase the content of immunoglobulin G in the pet cat's blood and the content of secretory immunoglobulin A in the feces.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compound lactic acid bacteria microecological preparation and a preparation method and application thereof. Background technique [0002] With the improvement of people's living standards, family pet cats have gradually become popular and popular from the niche exclusive use of some high-income groups in the past. Foreign cat breeds such as cats, Persian cats, Balinese cats and fold-eared cats can bring beneficial effects to people of different ages. For example, feeding pet cats to children can cultivate their love, sense of responsibility and independent thinking ability from an early age Feeding pet cats for middle-aged people can release the pressure of work and life to a certain extent, and relax; feeding pet cats for the elderly can alleviate loneliness, enrich leisure life, and improve physical and mental happiness. [0003] As a small omnivorous animal, both do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/40C12R1/25C12R1/245C12R1/01
CPCA23K10/18A23K50/40C12N1/20
Inventor 黄卫强乔向前
Owner INNER MONGOLIA SCI PLUS BIOTECH
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