Primer and probe for detecting c-MET gene amplification through digital PCR technology and detection method thereof

Abstract

The invention relates to molecular biology detection of genes in the technical field of biology, and particularly relates to a method for detecting c-MET gene amplification by utilizing a digital PCR(Polymerase Chain Reaction) technology. The detection method comprises the following steps: firstly, designing and synthesizing upstream and downstream primers for detecting c-MET gene amplification and the probe for detecting the c-MET gene amplification for c-MET genes; then designing digital PCR detection parameters; judging whether a sample to be detected contains c-MET gene amplification through the digital PCR technology. The primer and the probe designed by the invention have strong specificity, are applied to non-tumorous tissues through the digital PCR detection, the accuracy is high,and the sensitivity can be up to 0.01 percent. The length of an amplification fragment of the primer designed and synthesized by the method disclosed by the invention is less than 100bp, so that thefragment is particularly suitable for amplifying small-fragment DNA samples such as plasma free DNA, and variation of c-MET amplification with extremely low abundance ratio can be detected from cfDNA,tumor DNA is detected from blood circulation of patients in the early stage of tumors; moreover, the technical problem of risk of invasive sampling detection is overcome, and the primer, the probe and the detection method have extremely important effects on guidance of clinical treatment and improvement of prognosis of patients.

Description

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AI Technical Summary

Benefits of technology

In this new technology, there were advantages over previous methods but they had weaknesses or limitations during their use due to factors like poor targeting ability caused by hybridization between different sequences within the same sequence. These improvements led to improved accuracy and stability in diagnosing cancer types based on its presence at certain points along genome.

Problems solved by technology

This patents describes how certain type of nucleic acid called catheter endonuclease (CENE1) may cause various diseases such as gastrointestinal stomach adhesion disease or gastritis fibroblasts carcinoma. It also explains why these conditions are associated with increased risk of developing colorectomy related complications like death from colon cancer. Current diagnoses rely heavily upon histology analysis and immunohistochemistry, making them time-consuming processes and subject to errors.

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