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Kit and method for detecting anthurium andraeanum SERK gene expression status

A gene expression and kit technology, which is applied in the field of detecting the SERK gene expression status of flamingos, can solve the problems of staying at the level of morphological cytology and so on.

Inactive Publication Date: 2013-02-13
BEIJING UNIV OF AGRI
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Problems solved by technology

[0005] At present, most of the research in our country stays at the level of organogenesis pathway, and there are few reports on somatic embryogenesis pathway, and they mainly stay at the level of morphological cytology

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  • Kit and method for detecting anthurium andraeanum SERK gene expression status

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Embodiment Construction

[0016] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.

[0017] Take the young leaves just unfolded as explant materials, after surface sterilization with 0.1% mercuric chloride for 3 minutes, wash them with sterile water 4-5 times, cut the leaves along the midrib into 1×1cm pieces and inoculate them on the 1 / 2MS+0.5mg / L6-BA+0.5mg / L2, in 4-D medium, the pH value of the medium is 5.8, inoculate 10 bottles, 3 leaves per bottle, and store at (25±2)°C under dark conditions callus induction. Two months later, after the granular callus was connected into pieces, cut the callus and place it on 1 / 2MS+1.0mg / L6-BA+0.1mg / L KT+0.5mg / L NAA for subculture, subculture The newly obtained callus was cut and placed on 1 / 2MS+0.5mg / L6-BA medium...

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Abstract

The invention relates to a kit and a method for detecting an anthurium andraeanum SERK gene expression status, and belongs to the field of plant molecular biology. According to the invention, a just-expended young leaf of an anthurium andraeanum potted variety Alabama mature plant is adopted as an explant, and anthurium andraeanum embryogenic callus induction is carried out; non-embryogenic callus and embryogenic callus obtained in two induction stages are subjected to cytological observation; a full-length sequence of cDNA gene of anthurium andraeanum somatic embryo SERK gene is obtained by cloning; primers with relatively high specificity are designed; and researches and analysis upon changes of expression amount of SERK gene transcriptional level of anthurium andraeanum somatic embryo induction and development stages are carried out by using a real-time fluorescent quantitative PCR technology, such that a low-cost and high-specificity kit used for detecting anthurium andraeanum SERK gene expression status is obtained, and a method thereof is provided.

Description

technical field [0001] The invention relates to a method for gene detection, in particular to a method for detecting the expression status of the SERK gene of anthurines. Background technique [0002] Anthurium andraeanum, also known as Anthurium and Anthurium, is a plant of the genus Anthurium in the family Araceae, native to the tropical rain forests of South America. Its unique flower shape, bright and gorgeous spathes, rich colors, long flowering period, unique leaf shape, and excellent foliage and flower viewing are rare decorative treasures for living rooms. In the world flower trade, Anthurium is second only to tropical flowers. Orchid is the second largest tropical flower. It was introduced and cultivated in my country in the 1970s, and it has become a famous and precious cut flower variety. [0003] Generally, anthurines are commonly used to propagate by division, which is very slow. Occasionally, cuttings are used to reproduce, but the survival rate is very low. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 张克中崔金腾王爱香张睿鹂
Owner BEIJING UNIV OF AGRI
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