Primer set for detection of cytomegalovirus and its application

A technology of iridescent virus and primer set, which is applied in the field of primer set for detection of cytomegalovirus iridescent virus, can solve the problems of high mortality rate, insufficient sensitivity, and high cost of diseased fish, and achieve low instrument dependence, strong specificity, and cost saving the effect of time

Active Publication Date: 2021-03-02
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, fish diseases caused by cytomegalovirus iridescent virus have shown an obvious upward trend, and the mortality rate of diseased fish is high, causing huge economic losses to the aquaculture industry
[0003] At present, relevant researches on the detection methods of iridescent viruses have been carried out at home and abroad, but many methods are time-consuming, costly, require high equipment requirements, insufficient sensitivity, and low specificity; it is urgent to establish a fast, efficient, high-sensitivity , a highly specific method for detecting the virus

Method used

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  • Primer set for detection of cytomegalovirus and its application
  • Primer set for detection of cytomegalovirus and its application
  • Primer set for detection of cytomegalovirus and its application

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Embodiment 1

[0055] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0056] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. The following examples are just to illustrate the present invention, but not to limit the scope of the present invention in any way.

[0057] 1. Design and synthesis of primers

[0058] According to the gene sequences of multiple fish cell swelling iridescent viruses, the sequence numbers are KC775381.1, AY532608.1AY285746.1, AB263097.1, AB178942.1, through https: / / www.ebi.ac.uk / Tools / msa / clustalo / web site comparison, obtain a highly conserved sequence, its sequence is shown in SEQ ID No: 1, which is used as the sequence region of the primer design of the present invention.

[0059] Use PrimerExplorer V4 software to design LAMP primers according to the sequence SEQ ID No:1, select the Tm...

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Abstract

The present invention disclosed the primer group and its application of the cell swelling of the big rainbow virus, which belongs to the field of biological testing technology.The present invention provides a high -conservative region designed by a highly conservative region designed by a variety of fish cells to swelling the big rainbow virus gene sequence. This primer group has the characteristics of strong specificity and high sensitivity.The kit of the swollen big rainbow virus, and the LAMP method is used to detect the characteristics of the fish cell swelling and the red color virus.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a primer set for detecting cytomegalovirus iridescent virus and its application. Background technique [0002] Iridoviruses mainly infect invertebrates and lower vertebrates; they are large icosahedral, cytoplasmic DNA viruses. Iridoviruses are divided into 5 genera, namely Iridovirus, Chloriridovirus, Ranavirus, Lymphocystivirus and Megalocytivirus . Among them, cytomegalovirus genus iridovirus is one of the important viral pathogens of fish. In recent years, fish diseases caused by cytomegalovirus iridescent virus have shown an obvious upward trend, and the mortality rate of diseased fish is high, which has caused huge economic losses to the aquaculture industry. [0003] At present, relevant researches on the detection methods of iridescent viruses have been carried out at home and abroad, but many methods are time-consuming, costly, require high eq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/6844C12Q1/701
Inventor 葛辉林克冰黄种持周宸郑乐云林琪
Owner FISHERIES RES INST OF FUJIAN
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