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Bupleurum bicaule substituting composition for preparation of prostate cancer therapeutic drugs and determination method thereof

A technology for treating prostate cancer and drugs, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations containing active ingredients, and can solve problems such as unclear active ingredients, difficult quality, and complex ingredients.

Inactive Publication Date: 2018-08-24
杨真慧
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Using roots as medicine has the disadvantages of complex ingredients, unclear medicinal ingredients, and difficult quality control

Method used

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  • Bupleurum bicaule substituting composition for preparation of prostate cancer therapeutic drugs and determination method thereof
  • Bupleurum bicaule substituting composition for preparation of prostate cancer therapeutic drugs and determination method thereof
  • Bupleurum bicaule substituting composition for preparation of prostate cancer therapeutic drugs and determination method thereof

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Embodiment Construction

[0046] The substantive content of the present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention is not limited thereto.

[0047] 1. Experimental materials

[0048] Bupleurum chinensis was collected from Hailaer, Inner Mongolia, dried at 55°C overnight after crushing, and stored in a dry place for future use.

[0049] Agilent 1100 liquid chromatograph; Sartorius BS210S electronic balance, Beijing Sartorius Instrument System Co., Ltd.; Sartorius CP225D electronic balance, Beijing Sartorius Instrument System Co., Ltd.; acetonitrile (chromatographically pure) and methanol (chromatographically pure) were purchased from TEDIA; the water is Wahaha purified water.

[0050] RNAiso plus reagent, SYBRGreen PCR Master Mix and PrimeScript RT kit were purchased from TaKaRa.

[0051] 2. Experimental method

[0052] 1. Determination of saikosaponin F, saikosaponin G, saikosaponin I, s...

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Abstract

The invention discloses a bupleurum bicaule substituting composition for preparation of prostate cancer therapeutic drugs and a determination method thereof. The composition composed of bupleurum prosapogenin F, bupleurum prosapogenin I, bupleurum prosapogenin C, bupleurum prosapogenin A and bupleurum prosapogenin D basically can substitute the inhibitory effect of bupleurum bicaule on AMPK gene expression, and the two have equivalent intensity. The composition can be used for equivalent substitution of bupleurum bicaule to prepare AMPK gene expression inhibitors. The technical staff of the technical field are aware that inhibition of the AMPK gene expression can effectively inhibit the proliferation of prostate cancer cells (influence of RNA interference silencing of AMPK on the biological behaviors of prostate cancer cells, Chinese Journal of Cancer Prevention and Treatment, Volume 25, Issue 2, Jan. 2018). Therefore, the composition can be used for equivalent substitution of bupleurum bicaule to prepare prostate cancer therapeutic drugs.

Description

technical field [0001] The invention relates to a composition used to replace Bupleuri Coniferae to prepare tumor therapeutic drugs and an assay method thereof. Background technique [0002] Bupleurum bicaule Helm is the dried root of Bupleurum bicaule Helm in the genus Bupleurum bicaule Helm of the family Bupleurum. Saponins are the main active ingredients of Bupleurum genus medicinal plants. Modern pharmacological studies have shown that saikosaponins have anti-inflammatory, analgesic and sedative, liver-protecting, anti-depressant, and anti-cancer effects. [0003] Using roots as medicine has the disadvantages of complex components, unclear efficacy components, and difficult quality control. If a composition that can replace Bupleuri Coniferae as medicine can be found, a chemical blend can be used instead of Bupleurum Coniferae as medicine, with clear ingredients and controllable quality. [0004] Bupleurum chinensis mainly contains saikosaponin F, saikosaponin G, saiko...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/704A61P13/08A61P35/00G01N30/74
CPCA61K31/7048A61K31/704A61P13/08A61P35/00G01N30/74A61K2300/00
Inventor 杨真慧
Owner 杨真慧
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