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Method for detecting residue of veterinary drugs in aquatic products

A technology for veterinary drug residues and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of veterinary drug residues, cumbersome operation process, large amount of extractant, etc., and achieve low detection limit and quantification limit, high linear correlation performance, and the effect of reducing detection impact

Pending Publication Date: 2018-10-16
孝感寰誉新材科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The output of aquatic products in my country is constantly increasing. In order to prevent and treat aquatic product diseases and promote the growth of aquatic products, a variety of veterinary drugs will be used in the breeding process, even banned and restricted drugs, which will result in varying degrees of veterinary drug residues; veterinary drugs in aquatic products Residues have the characteristics of various types, multiple analytical components, complex matrix components, and low levels of veterinary drug residues. The above inventions and existing technologies are not specifically aimed at the detection of veterinary drug residues in aquatic products, and there are large amounts of extractants, cumbersome operations, and poor stability. , poor repeatability and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for detecting veterinary drug residues in aquatic products, the technical scheme of which is as follows:

[0021] Step 1. Extraction of residual veterinary drugs: Homogenize the aquatic product sample; then weigh 2.5g of the homogenized aquatic product and place it in a clean centrifuge tube, then add 0.8ml of acetic acid and 13ml of solvent, vortex at the maximum speed for 110s; then Centrifuge at a speed of 15000r / min for 280s at 13°C; take out 6ml of the supernatant after stopping the centrifugation, add 5 parts of terbium-doped lipid adsorbent, vortex at the maximum speed for 110s, then let it stand for 13min, and then use 15000r / min Centrifuge at a speed of 280s; after stopping the centrifugation, take out 4.8ml of the supernatant, add 6 parts of saturated saline, vortex at the maximum speed for 110s, and then centrifuge at a speed of 15000r / min for 280s; take 5ml of the supernatant, and pass through a microporous membrane;

[0022] Step 2. Detection of re...

Embodiment 2

[0030] A method for detecting veterinary drug residues in aquatic products, the technical scheme of which is as follows:

[0031]Step 1. Extraction of residual veterinary drugs: Homogenize the aquatic product sample; then weigh 2.0 g of the homogenized aquatic product and place it in a clean centrifuge tube, then add 0.5 acetic acid and 10 ml of solvent, vortex at the maximum speed for 100 seconds; Centrifuge at 10000r / min for 250s; stop centrifugation and take out 5.5ml of supernatant, add 2.5 parts of terbium-doped lipid adsorbent, vortex at maximum speed for 100s, then let stand for 10min, and then use 10000r / min Centrifuge at a speed of 250s; after stopping the centrifugation, take out 4.5ml of the supernatant, add 5.5 parts of saturated saline, vortex at the maximum speed for 100s, and then centrifuge at a speed of 10000r / min for 250s; take 2.5ml of the supernatant, and pass through a microporous membrane;

[0032] Step 2. Detection of residual veterinary drugs: 1) Liquid...

Embodiment 3

[0040] A method for detecting veterinary drug residues in aquatic products, the technical scheme of which is as follows:

[0041] Step 1. Extraction of residual veterinary drugs: Homogenize the aquatic product sample; then weigh 3.0g of the homogenized aquatic product and place it in a clean centrifuge tube, then add 1.0ml of acetic acid and 15ml of solvent, vortex at the maximum speed for 120s; then Centrifuge at 15°C for 300s at a speed of 20,000r / min; take out 6.5ml of the supernatant after stopping the centrifugation, add 3.5 parts of terbium-doped lipid adsorbent, vortex at the maximum speed for 120s, then let stand for 15min, and then use 20,000r / min Centrifuge at a speed of 300s; after stopping the centrifugation, take out 5.0ml of the supernatant, add 6.5 parts of saturated saline, vortex at the maximum speed for 120s, and then centrifuge at a speed of 20000r / min for 300s; take 3.5ml of the supernatant, and pass through a microporous membrane ;

[0042] Step 2. Detect...

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PUM

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Abstract

The invention relates to the field of food detection, in particular to a method for detecting residue of veterinary drugs in aquatic products. The method provided by the invention is used for detecting the residual veterinary drugs in the aquatic products. Compared with the existing detection methods, the method has higher accuracy, can simultaneously extract, purify and test the various veterinary drugs, and is fast and efficient. The method provided by the invention adopts a terbium-doped lipid adsorbent, can absorb the residual grease in the aquatic products during the extraction of the veterinary drugs, reduces the influence of the grease on the detection of the veterinary drugs, and improves the accuracy, sensitivity and precision of the detection. The analytical method provided by the invention has very high linear correlation, accuracy and purification effect, and is lower in detection limit and limit of quantitation.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a detection method for veterinary drug residues in aquatic products. Background technique [0002] In recent years, food safety issues have been paid close attention to by people. With the transformation of people's demand for animal-sourced foods to quality-based ones, veterinary drug residues in animal-sourced foods have gradually become a focus of attention all over the world. The Joint Expert Committee on Food Additives and Contaminants began to evaluate the toxicity of veterinary drug residues in the 1960s, providing a scientific basis for people to understand the hazards of veterinary drug residues and their control. [0003] CN107505373A discloses a biosensor electrode for detecting residues of various veterinary drugs, which includes a rod-shaped electrode carrier made of organic insulating material, and the lower end cross-section of the rod-shaped electrode carrier is coa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王艺霖胡妍
Owner 孝感寰誉新材科技有限公司
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