Detection card for forward and reverse ABO blood typing and RhD blood typing

A positive and negative stereotype and detection card technology, which is applied in biological testing, material inspection products, etc., can solve the problems of non-specific adsorption of miscellaneous proteins and red blood cells, poor sensitivity and specificity, and low detection accuracy, so as to prevent denaturation or occurrence of non-specific Normal agglutination, strong objectivity, and the effect of eliminating interference factors

Inactive Publication Date: 2018-11-16
河北鑫辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some manufacturers at home and abroad have produced ABO positive and negative typing and RhD blood type test cards, but there are some shortcomings: dextran gel or polypropylene dextran gel is used as a filler, and its sensitivity and specificity are poor, resulting in

Method used

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  • Detection card for forward and reverse ABO blood typing and RhD blood typing
  • Detection card for forward and reverse ABO blood typing and RhD blood typing

Examples

Experimental program
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Example Embodiment

[0053] Example 1

[0054] The detection card of the present invention comprises 8 microcolumns, adopts following steps to prepare ABO positive and negative typing and RhD blood type typing detection card:

[0055] (1) Prepare antibody diluent and anti-fix buffer; the composition of antibody diluent and anti-fix buffer is the same, and the formulas of antibody diluent and anti-fix buffer are shown in Table 1;

[0056] (2) Dilute the anti-A antibody, anti-B antibody, and anti-D antibody with antibody diluent respectively to anti-A antibody solution, anti-B antibody solution, and anti-D antibody solution with a titer of 1:64-1:256;

[0057] (3) Add 15 microliters of anti-A antibody solution, anti-B antibody solution, anti-D antibody solution, and antibody diluent to 1-4 microcolumns in sequence, and add 15 microliters of anti-fixation buffer to 5-8 microcolumns respectively;

[0058] (4) Add 30 mg of glass beads to 1-8 microcolumns respectively;

[0059] (5) Heat and seal with ...

Example Embodiment

[0061] Example 2

[0062] The detection card of the present invention comprises 8 microcolumns, adopts following steps to prepare ABO positive and negative typing and RhD blood type typing detection card:

[0063] (1) Prepare antibody diluent and anti-fix buffer; the composition of antibody diluent and anti-fix buffer is the same, and the formulas of antibody diluent and anti-fix buffer are shown in Table 1;

[0064] (2) Dilute the anti-A antibody, anti-B antibody, and anti-D antibody with antibody diluent respectively to anti-A antibody solution, anti-B antibody solution, and anti-D antibody solution with a titer of 1:64-1:256;

[0065] (3) Add 25 microliters of anti-A antibody solution, anti-B antibody solution, anti-D antibody solution, and antibody diluent to 1-4 microcolumns in sequence, and add 25 microliters of anti-fixation buffer to 5-8 microcolumns respectively;

[0066] (4) Add 50 mg of glass microspheres to 1-8 microcolumns respectively;

[0067] (5) Heat and sea...

Example Embodiment

[0069] Example 3

[0070] The detection card of the present invention comprises 8 microcolumns, adopts following steps to prepare ABO positive and negative typing and RhD blood type typing detection card:

[0071] (1) Prepare antibody diluent and anti-fix buffer; the composition of antibody diluent and anti-fix buffer is the same, and the formulas of antibody diluent and anti-fix buffer are shown in Table 1;

[0072] (2) Dilute the anti-A antibody, anti-B antibody, and anti-D antibody with antibody diluent respectively to anti-A antibody solution, anti-B antibody solution, and anti-D antibody solution with a titer of 1:64-1:256;

[0073] (3) Add 20 microliters of anti-A antibody solution, anti-B antibody solution, anti-D antibody solution, and antibody diluent to 1-4 microcolumns in sequence, and add 20 microliters of anti-fixation buffer to 5-8 microcolumns respectively;

[0074] (4) Add 40 mg of glass beads to 1-8 microcolumns respectively;

[0075] (5) Heat and seal with ...

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Abstract

The invention discloses a detection card for forward and reverse ABO blood typing and RhD blood typing, belonging to the field of blood typing. The detection card of the invention comprises 8 micro-columns. A preparation method for the detection card comprises the following steps: (1) preparing an antibody diluent and a reverse typing solution; (2) respectively diluting an anti-A antibody, an anti-B antibody and an anti-D antibody with the antibody diluent into an anti-A antibody solution, an anti-B antibody solution and an anti-D antibody solution with titer of 1: 64 to 1: 256; (3) sequentially add 15-25 microliters of the anti-A antibody solution, the anti-B antibody, the anti-D antibody solution and the antibody dilutent into the micro-columns No. 1 to No. 4 and separately adding the reverse typing solution into the micro-columns No. 5 to No. 8; (4) separately adding glass beads into the 8 micro-columns; (5) and heating aluminum foils to seal the micro-columns. The detection card ofthe invention has good stability in agglutination reactions, is highly objective and accurate in result determination, and effectively prevents the phenomenon of misjudgement; and the effective timeof result interpretation may be a plurality of hours, which effectively prevents interpretation errors caused by improper control of operation time.

Description

technical field [0001] The invention relates to the field of blood type typing testing, in particular to a test card for ABO positive and negative typing and RhD blood typing. Background technique [0002] The discovery of ABO blood type solved the basic problem of blood transfusion and laid the foundation for the rapid development of medical science. Today, blood group serology is still an important topic in blood transfusion research, and blood group identification and cross-matching are the core technologies to ensure blood transfusion safety. [0003] Positive typing belongs to cell antigen typing, and reverse typing belongs to serum antibody typing. Using positive type alone to identify ABO blood type may cause misjudgment in some cases. Misjudgment of ABO blood type is extremely risky for clinical blood transfusion, which will cause ABO blood type abnormal blood transfusion, easily lead to the occurrence of acute hemolytic transfusion reaction, and pose a threat to t...

Claims

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Application Information

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IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 王振华李胜斌刘春辉
Owner 河北鑫辉生物科技有限公司
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