A system and method for dividing and counting aggregated leukocytes

Abstract

The invention discloses a system for dividing and counting aggregated leukocytes. The system includes: an image acquisition module for staining leukocytes in a blood sample, using erythrocyte lysate to dissolve the red blood cells in the blood sample, and obtaining images of leukocytes; an image preprocessing module , for performing image background removal on the white blood cell image and using the maximum inter-class variance method to obtain the optimal segmentation threshold, and roughly segment the white blood cell area; the aggregated cell judgment module is used for obtaining a rough segmentation image according to the rough segmentation of the white blood cell area, set The discriminant function of the cell area is determined to obtain the multi-cell aggregation area; the aggregated cell segmentation and counting module is used to extract the cytoskeleton in each aggregation area and the gray-scale curve at the cytoskeleton by using the morphological refinement method. In the present invention, by analyzing the gray scale characteristics of various types of white blood cells under a low-power microscope, an adaptive threshold function is constructed to obtain the number of eosinophils while obtaining the count of white blood cells, and quickly and accurately segment and count the cells in the aggregation area. Simple and easy to implement.

Description

technical field [0001] The present invention relates to a system and method for dividing and counting white blood cells, in particular to a system and method for dividing and counting aggregated white blood cells. Background technique [0002] White blood cell detection is an important part of clinical examination, and its content and quality are important indicators of human health. Automatic instrument analysis and manual microscopic examination are the main methods for leukocyte classification and counting at present, but they still have the disadvantages of bulky equipment, difficult maintenance and highly professional operation, which are not suitable for primary medical and health institutions, mobile medical and home use. The image-based portable white blood cell counter can obtain accurate diagnostic information by analyzing blood sample images at the place closest to the patient, which has time and space advantages that large-scale instruments do not have. However,...

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to overcome the deficiencies of the prior art, the present invention provides a system and method for dividing and counting aggregated white blood cells, which can solve the problem of inability to obtain accurate cell segmentation and complete separation of aggregated cells, especially highly aggregated cells, and the accuracy of counting low problem

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