Multi-stage acoustophoresis device
An acoustophoresis, station-type technology, applied in biochemical cleaning devices, enzymology/microbiology devices, fluids using vibration, etc., can solve fluid flow restrictions, limited efficacy of acoustophoresis devices, and inability to capture different types of materials, etc. question
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Embodiment 1
[0127] Compare the acoustophoretic separation process with deep flow filtration (DFF). First, a DFF capacity baseline was obtained by conducting two rounds of clarification, primary clarification and secondary clarification. The settings for this baseline are shown in Figure 13 .
[0128] The pressure drop was measured during the two runs. Separation equipment at 145LMH (liter / m 2 / hour) operation. Pressure is measured at three different locations P1, P2 and P3. Filters are located between each set of sensors. The filters used during primary clarification were DOHC filters and the filters used during secondary clarification were XOHC filters, both available from Millipore.
[0129] The mixture of CHO cells and medium flows through the filter, and the permeate is collected in a tank. CHO cells were removed through a filter. The feed has a 6.34 x 10 6 Total cell density (TCD) of cells / mL and turbidity of 815 NTU. The final permeate in the third tank had a turbidity o...
Embodiment 2
[0132] Next, the two-step DFF described in Example 1 was compared to a two-step clarification process in which primary clarification was performed by acoustic wave separation (AWS) and secondary clarification was performed by DFF. This comparison is set as Figure 14 shown.
[0133] Such as Figure 14 As shown, in the two-step DFF, each filter has 11m 2 area. Each filter was operated with a pressure drop of 7.5 psig. For each filter, 7.5psig (VT 7.5 ) under the volume throughput (VT) is 84L / m 2 .
[0134] The acoustophoresis system used to perform AWS consisted of three acoustophoresis devices connected in series. The transducers in each device are 1 inch by 1 inch. The system has a 49cm 3 total sound volume. AWS system with a total area of 6m 2 DFF filter pairing. Since there is no pressure drop in the AWS system, the DFF filter can operate at 15psig pressure drop and produce 160L / m 2 VT 15 .
[0135]The feed has a 6.7 x 10 6 Total cell density (TCD) of cell...
Embodiment 3
[0140] The same experiment as described in Example 2 was performed again, but with a higher cell density. The feed has a 15.6 x 10 6 Total TCD in cells / mL and turbidity of 3608 NTU and cell viability of 68%. This comparison is set as Figure 15 mentioned.
[0141] The two-step DFF process uses 38m respectively 2 and 17m 2 filter. As indicated, the primary clarified VT 7.5 is 26L / m 2 , the secondary clarification is 58L / m 2 . In the AWS-DFF process, the AWS system has two acoustophoresis devices connected in series (instead of the three in Example 2), and the total acoustic volume is 33 cm 3 . DFF filter has 11m 2 The total area and 85L / m 2 VT 15 . The feed rate of the acoustophoresis system was 8 kg, 2.5 liters per hour (LPH).
[0142] The results of primary clarification using the AWS system are as follows Figure 15 mentioned. The acoustophoresis system achieved 94% TCD reduction, 91% turbidity reduction and 92% protein recovery. The graph on the lower left...
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