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Liquid chromatography-mass spectrometry combined metabolite detection method

A liquid chromatography and detection method technology, which is applied in the field of substance detection, can solve problems such as exceeding the detection threshold of equipment and excessive sample content, and achieve the effects of reducing data analysis and time, improving experimental efficiency, and ensuring accuracy and effectiveness

Inactive Publication Date: 2019-01-11
嘉兴迈维代谢生物科技有限公司
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Problems solved by technology

[0003] In the detection by liquid chromatography-mass spectrometry system, due to the uncertain performance of the content of the substance in the sample, it is often prone to the phenomenon that the sample content is too high to exceed the detection threshold of the equipment

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  • Liquid chromatography-mass spectrometry combined metabolite detection method
  • Liquid chromatography-mass spectrometry combined metabolite detection method
  • Liquid chromatography-mass spectrometry combined metabolite detection method

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[0039] In order to make the above objects, features and advantages of the present invention more comprehensible, the specific implementation of the present invention will be described in detail below in conjunction with specific examples.

[0040] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0041]Second, "one embodiment" or "an embodiment" referred to herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. "In one embodiment" appearing in different places in this specification does not all refer to the same embodimen...

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Abstract

The invention discloses a liquid chromatography-mass spectrometry combined metabolite detection method, comprising the following steps: performing first online detection on a sample to be detected; introducing a standard product detection database file; preparing first detection result offline data of the sample to be detected; preparing DP and CE combined gradient information; setting a peak height threshold; deriving DP and CE optimization results; establishing a DP and CE optimization database according to optimized detection results. According to the method, the DPCE optimization conditionanalysis and the time consuming of a conventional project are greatly reduced. The sample collection situation is accurately monitored by using 2-chlorophenylalanine as a general internal standard, and the fluctuating state of an instrument is monitored by using 17 mixed standards as general quality control products. The data analysis and time are greatly reduced, accurate monitoring of the collection process and the instrumental fluctuation is realized, and the experimental efficiency of liquid chromatography-mass spectrometry series detection is improved.

Description

technical field [0001] The invention belongs to the technical field of substance detection, and in particular relates to a liquid chromatography-mass spectrometry detection method for metabolites. Background technique [0002] Metabolomics is a discipline similar to genomics and proteomics, which conducts large-scale qualitative and quantitative analysis of metabolites in organisms, and uses this to find out the relationship between each metabolite. [0003] In the detection by liquid chromatography-mass spectrometry system, due to the uncertainty of the content of substances in the sample, it is often prone to the phenomenon that the sample content is too high to exceed the detection threshold of the equipment. By adjusting the declustering potential (Declustering Potential, DP) and collision energy (collision Energy, CE) of each target substance, the threshold value of the detection substance is adjusted to the effective detection range. The adjustment of DP and CE not on...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 唐堂高猛董学奎王宏郑彬石坚
Owner 嘉兴迈维代谢生物科技有限公司
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