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A method for preparing mink distemper antigen protein complex, antigen protein complex and application thereof

A canine distemper virus and mink technology, which is applied in the field of biopharmaceuticals, can solve the problems of long complex cycle, unstable quality, large batch differences, etc., and achieve the effects of artificial reduction, easy amplification and cultivation, and shortened cycle.

Active Publication Date: 2022-04-12
JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the cumbersome and complicated process of producing the mink distemper antigen protein complex, the long cycle, high labor intensity, laborious work, unstable quality, large batch-to-batch variation, and high side effects after the prepared virus antigen complex is injected into animals, etc. , the present invention provides a method for preparing mink distemper antigen protein complex by using low serum suspension culture VeroE6-TY strain cells, antigen protein complex and application thereof

Method used

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  • A method for preparing mink distemper antigen protein complex, antigen protein complex and application thereof
  • A method for preparing mink distemper antigen protein complex, antigen protein complex and application thereof
  • A method for preparing mink distemper antigen protein complex, antigen protein complex and application thereof

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Experimental program
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Embodiment 1

[0048] 1. Recovery of VeroE6-TY strain cells

[0049] Take the VeroE6-TY strain suspension culture cells frozen in liquid nitrogen, place them in a 37°C water bath to thaw quickly and inoculate them in a shake flask containing 300ml of low serum suspension culture medium, place them at 37°C, 60rpm, 5% (v / v) CO 2 Cultured on a shaker for 72 hours, the cell density can reach 2.0×10 6 cells / ml, cell viability ≧90%, and recovered VeroE6-TY strain cells were obtained.

[0050] 2. Low-serum culture medium for amplified culture of VeroE6-TY strain cell culture medium

[0051] A) Inoculate the recovered VeroE6-TY strain cells obtained in step 1 into shake flasks (3 shake flasks) with low serum culture medium at a volume ratio of 1:3 to the culture medium, and supplement each bottle with low serum culture solution to 300ml at 37°C 5% CO 2 Cultivate on a shaker for 72 hours, 60r / min. The number of suspended cells is not less than 1.5×10 6 cells / ml, cell viability≧90%, obtained th...

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Abstract

The invention relates to a method for preparing mink canine distemper antigen protein complex, the antigen protein complex and application thereof, belonging to the field of biopharmaceuticals. The domesticated mink distemper virus was cultured by suspending VeroE6-TY strain cells in low-serum medium, and the domesticated virus was inoculated into the low-serum suspension VeroE6-TY strain cell culture medium, and the venom was harvested after culture and proliferation to prepare mink distemper antigen protein Complex. The invention realizes the transformation of the mink distemper antigen protein complex from spinner bottle adherent culture to large-scale cell suspension culture, which not only simplifies the production process, reduces production cost, but also greatly improves the yield and quality of products.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a preparation method of an antigen-protein complex, an antigen-protein complex and applications thereof. Background technique [0002] Mink distemper is an acute, highly contagious disease of mink caused by canine distemper virus. It mainly affects the respiratory system, digestive system and nervous system, and is characterized by typical bidirectional heat and catarrhal inflammation of the upper respiratory tract, lung and gastrointestinal tract. One of the three most harmful infectious diseases in fur animal breeding has brought huge economic losses to the breeding industry. VeroE6 cells are African green monkey kidney passage cells, which are widely used in the research of virus antigen preparation. Traditional VeroE6 cells are anchorage-dependent growth cells. When producing vaccines, 10 liters or 15 liters of spinner bottles are used to adhere to culture cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/175A61P31/14
CPCA61K39/12C12N7/00C12N2760/18434C12N2760/18451A61K2039/552
Inventor 任飞王春霞王彬叶明闫如勋刘秉玺李云松倪佳王萃瑜许皓
Owner JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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