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A method for preparing mink parvovirus enteritis antigen protein complex, antigen protein complex and application thereof

A technology for toxic enteritis and minor diseases, applied in the field of biopharmaceuticals, can solve problems such as large batch differences, unstable quality, and long complex cycle, and achieve the effect of shortening production cycle and uniform and stable product quality.

Active Publication Date: 2021-11-30
JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the cumbersome and complicated process of producing the mink parvovirus enteritis antigen protein complex, the process is long, labor intensive, labor intensive, and the quality is unstable, the difference between batches is large, and the prepared virus antigen complex has high side effects after injection into animals, etc. Problem, the present invention provides a method for preparing mink parvovirus enteritis antigen protein complex by using low serum suspension culture F81-TY strain cells, antigen protein complex and its application

Method used

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  • A method for preparing mink parvovirus enteritis antigen protein complex, antigen protein complex and application thereof
  • A method for preparing mink parvovirus enteritis antigen protein complex, antigen protein complex and application thereof
  • A method for preparing mink parvovirus enteritis antigen protein complex, antigen protein complex and application thereof

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Experimental program
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Embodiment 1

[0055] 1. Recovery of F81-TY strain cells

[0056] Take F81-TY strain low-serum suspension culture cell cryopreservation tubes cryopreserved in liquid nitrogen, and the number of cells in each cryopreservation tube should be at least 3.0×10 6 cells / mL, quickly thawed in a 37°C water bath and inoculated with 400 ml of low-serum culture medium containing 1-5% bovine serum by volume, placed in a shake flask at 37°C in 5% CO 2 Cultivate on a shaker for 72 hours, 60r / min. After culturing for 72 hours, the revived F81-TY strain cells were obtained, and the number of suspended cells could reach 3.0×10 6 individual / mL.

[0057] 2. Low-serum culture medium for amplified culture of F81-TY strain cell culture medium

[0058] A) The suspension cells obtained in step 1 were subcultured in 4 shake flasks according to the volume ratio of 1:4 to the culture medium. 2 Cultivate on a shaker for 72 hours, 60r / min. The number of suspended cells is not less than 3.0×10 6 , cell viability ≧ 9...

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Abstract

The invention relates to a method for preparing mink parvovirus enteritis antigen protein complex, the antigen protein complex and application thereof, belonging to the field of biopharmaceuticals. The domesticated mink parvovirus enteritis virus was cultured by suspending F81-TY strain cells in low-serum medium, and the domesticated virus was inoculated into the low-serum suspension F81-TY strain cell culture medium, and the venom was harvested after culture and proliferation to prepare mink parvovirus enteritis virus Antigen protein complex. The invention realizes the transformation of mink parvovirus enteritis antigen protein complex from spinner bottle adherent culture to large-scale cell suspension culture, which not only simplifies the production process, reduces production cost, but also greatly improves the yield and quality of products .

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a preparation method of an antigen protein complex, the antigen protein complex and applications thereof. Background technique [0002] Mink parvovirus enteritis is an acute, severe and highly contagious infectious disease of mink caused by parvovirus, which is mainly characterized by severe diarrhea. One of the three most harmful infectious diseases in fur animal farming. Bring huge economic loss to aquaculture. F81 cells are cat kidney passage cells, which are widely used in the research of virus antigen preparation. Traditional F81 cells are anchorage-dependent growth cells. When producing vaccines, 10-liter or 15-liter spinner bottles are used to adhere to culture cells and then prepare virus antigen complexes. When producing the mink parvovirus enteritis antigen protein complex, first use the cell culture medium containing 10% newborn bovine serum to subcultu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/23A61P31/20
CPCA61K39/12A61K2039/552C12N7/00C12N2750/14034C12N2750/14051
Inventor 任飞王春霞王彬叶明闫如勋席娜张亭亭吕文雪王勇王玉明
Owner JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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