52 results about "Low-Serum Media" patented technology

The invention discloses a low-serum culture medium for high-density suspension culture of BHK-21 cells and an application of the low-serum culture medium in proliferation of FMDVs (foot and mouth disease viruses). According to researches on the metabolism level and mutual adjustment of nutritional substances such as glucose and the like in the growth process of the BHK-21 cells, the low-serum culture medium for individual BHK-21 cells is provided and comprises an amino acid part, a vitamin part, a balanced salt part and other additive parts. The culture medium is used for high-density suspension culture of the BHK-21 cells, the effective utilization rate of the nutritional substances by the cells can be greatly increased, the concentration of metabolism byproducts is reduced, and accordingly, the cell culture efficiency is improved; besides, the BHK-21 cells cultured by the culture medium have increased sensitivity for different serums of animals suffering from FMDV, the content of effective antigens 146S of the viruses is substantially increased, the purposes of high cell growth efficiency, cell growth controllability and minimization of the concentration of the metabolism byproducts are achieved, the production efficiency of biological products is higher, the production cost is lower, and the low-serum culture medium has remarkable economic benefits.