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Composition comprising stem cell active substance and method for preparing same

A technology of active substances and compositions, applied in the field of kits and cells and cell culture, can solve the problems of cell lethality, achieve the effect of reducing damage, reducing adverse reactions, and enhancing the effect

Active Publication Date: 2019-01-25
厦门神雁再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] However, in the aforementioned prior art, the great difficulty encountered during cryopreservation of cells is the cell lethality of the freezing and thawing (i.e., freeze-thaw) process, which poses a great challenge to the stem cell cryopreservation protocol and business

Method used

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  • Composition comprising stem cell active substance and method for preparing same
  • Composition comprising stem cell active substance and method for preparing same
  • Composition comprising stem cell active substance and method for preparing same

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Embodiment 1

[0070] Take fresh human-derived adipose-derived mesenchymal stem cells (obtained from Shandong Province Qilu Stem Cell Engineering Co., Ltd.), and its morphology micrograph is as follows: figure 1 shown. The stem cells were added to a cryoprotectant consisting of 1.0 wt.% DMSO (cell culture medium, purchased from Sigma Company), 2.0 wt.% glutathione (obtained from Beijing Biolab Company) , 2.0 wt.% trehalose (from Sinopharm Chemical Reagent Co., Ltd.) and 95 wt.% fetal bovine serum (FBS) (from Shanghai Yubo Biotechnology Co., Ltd.), the stem cells are 5 × 10 6 Each, the cryoprotectant is 2.0ml, cryopreserved for 15 months. After the end of the storage period, the conventional thawing method was used to thaw, and the morphology was observed with a microscope, and the morphology was as follows: figure 2 shown.

[0071] It was observed that after the cryoprotectant of the present invention was used for cryopreservation of the stem cells, even after 15 months of preservation,...

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Abstract

The invention provides a composition containing stem cell active substance and a preparation method thereof, wherein the composition comprises stem cells and a culture medium, wherein the culture medium is a low-serum culture medium or a serum-free culture medium. A kit comprising the composition is also disclosed. The composition comprising the stem cell active substance has high freeze-thaw reliability.

Description

technical field [0001] The invention belongs to the technical field of kits, cells and cell culture, and relates to a composition containing stem cell active substances and a preparation method thereof, in particular to a composition containing stem cell active substances, a kit containing the composition and a preparation method thereof. Background technique [0002] Stem cells have the potential to proliferate and differentiate, and are capable of self-renewal and replication, capable of producing highly differentiated functional cells. The original cells of various tissues and organs of animals. [0003] Due to the above characteristics of stem cells, it has a wide range of applications. At present, stem cells have been used to prepare reagents or kits that can be applied to various diseases, or to prepare health care reagents or kits. [0004] Mesenchymal stem cells from different sources hold great potential in the development of new therapies using stem cells. For ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0667C12N2500/90A01N1/0226C12N2500/44C12N2500/32C12N2501/125C12N2501/115C12N2501/135C12N2501/15
Inventor 周勇王全立陈海旭耿培亮
Owner 厦门神雁再生医学科技有限公司
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