Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells

A CH-1R, low serum technology, applied in the field of veterinary biology, can solve the problems such as no, and achieve the effects of reducing production costs, rapidly stabilizing production scale, and expanding production scale

Active Publication Date: 2015-05-06
成都史纪生物制药有限公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no relevant literature report on the production of porcine reproductive and respiratory syndrome CH-1R strain virus by culturing Marc-145 cells in low serum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells
  • Method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells
  • Method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the method that low serum culture Marc-145 cell produces porcine reproductive and respiratory syndrome CH-1R strain virus, it may further comprise the steps:

[0035] S1. Subculture and culture of cells for preparation:

[0036]Take a T75 flask and culture a monolayer of Marc-145 cells, digest the cells with EDTA-trypsin cell dispersion solution, the EDTA-trypsin cell dispersion solution is a mixture of 0.15% trypsin and 0.02% EDTA in Hank's solution Blow and beat the dispersed cells with cell growth medium, add 20ml of cell growth medium, place Marc-145 cells in a carbon dioxide incubator at a temperature of 37±2°C for 72 hours, and perform amplified culture when a good cell monolayer is formed; The cell growth medium is DMEM and newborn bovine serum, the volume ratio of DMEM and newborn bovine serum is 9:1, and the content of serum is 10%;

[0037] S2. Adapting cells to low serum medium: including the following sub-steps:

[0038] S21. Domestication of...

Embodiment 2

[0050] Embodiment 2: the method that low serum culture Marc-145 cell produces porcine reproductive and respiratory syndrome CH-1R strain virus, it comprises the following steps:

[0051] S1. Subculture and culture of cells for preparation:

[0052] Take a T75 flask and culture a monolayer of Marc-145 cells, digest the cells with EDTA-trypsin cell dispersion solution, the EDTA-trypsin cell dispersion solution is a mixture of 0.15% trypsin and 0.02% EDTA in Hank's solution solution, blow and disperse cells with cell growth solution, add 20ml of cell growth solution, place Marc-145 cells in a carbon dioxide incubator at a temperature of 37±2°C for 72 hours, and perform amplified culture when a good cell monolayer is formed; The cell growth medium is a mixture of MEM and newborn bovine serum, the volume ratio of MEM and newborn bovine serum is 9:1, and the content of serum is 10%;

[0053] S2. Adapting cells to low serum medium: including the following sub-steps:

[0054] S21. D...

Embodiment 3

[0066] Example 3: Effect of low serum medium on acclimating Marc-145 cells

[0067] Use T75 cell flasks, 37 ℃ static culture adapted to the fourth generation of Marc-145 cells in low serum medium, the ratio of serum addition is 1%, 2%, 3%, 4%, 5% five gradients, and the serum content used is set to The Marc-145 cells cultured in 10% DMEM was used as the control group, and the results were as follows: figure 1 , figure 2 , image 3 , Figure 4 , Figure 5 , Figure 6 See Table 1 for the indicators during the cell culture process.

[0068] Table 1: Effects of MD series low-serum medium on acclimatization of Marc-145 cells

[0069]

[0070] It can be seen from Table 1 that the average cell yield, cell activity and average specific growth rate of the experimental group were higher than those of the control group.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells, and belongs to the technical field of biology of veterinaries. The method comprises the following steps: S1. performing passage and culture of cells; S2. domesticating the cells to enable the cells to be adapted for low seroculture bases; S3.domesticating the cells to enable the cells to be adapted for low seroculture environment; S4. propagation of cell virus seeds. The method for producing propagation and respiratory tract syndrome CH-1R strain viruses for pigs by seroculturing Marc-145 cells, provided by the invention, can reduce the production cost obviously, besides, can improve the downstream purifying efficiency, and can rapidly and stably extend the production scale. Through the adoption of the method, balancing and stabilizing the quality is easy to realize.

Description

technical field [0001] The invention belongs to the technical field of veterinary biology, and in particular relates to a method for producing porcine reproductive and respiratory syndrome CH-1R strain virus by cultivating Marc-145 cells with low serum. Background technique [0002] At present, the production technology of animal vaccines in my country is relatively backward. Most of the cells used in the production of viruses come from spinner bottle culture, and the most basic synthetic cell culture medium is mainly used, and even natural medium (such as hydrolyzed milk protein) is added with about 10% newborn bovine serum , The problems of vaccine safety, quality and cost brought about by these animal-derived ingredients have been highlighted. The main manifestations are: ①The large amount of bovine serum makes the growth of cells highly dependent on serum, but serum is a mixture of uncertain components, and there are differences in components between batches, which direct...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N5/073C12R1/93
Inventor 徐宏军胡来根牟和平岳丰雄舒经香
Owner 成都史纪生物制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com