Low serum medium for cultivating human mesenchymal stem cells

Abstract

The invention relates to a low serum medium for cultivating human mesenchymal stem cells. The medium comprises the following components: 1-100[mu]g / ml of recombinant human insulin, 1-50ng / ml of basic fibroblast growth factor, 1-50[mu]g / ml of human transferrin, 1-80[mu]g / ml of seleninic acid, 1-300[mu]g / ml of serine, 1-200[mu]g / ml of choline chloride, 1-60[mu]g / ml of adenine, 1-100[mu]g / ml of single acetamide, 0.01-10mmol / ml of o-phosphorylethanolamine 1-50pg / ml of three iodine thyroid, 1-50[mu]l / ml of fetal calf serum. The medium has the characteristics of maintenance of good stem cell characters and high cell proliferation capability; and the medium reduces dependence of stem cells for fetal calf serum, reduces bad influences of the fetal calf serum for stem cell characteristics and differentiation, reduces fetal calf serum, and accords with clinic applications.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a low-serum medium for mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells [mesenchymal stem cells, MSC] is an important member of the stem cell family, derived from the mesoderm and ectoderm in the early development, and belongs to pluripotent stem cells. MSC was first discovered in the bone marrow because of its multi-directional differentiation potential, hematopoietic Supporting and promoting stem cell engraftment, immune regulation and self-replication have attracted increasing attention. For example, mesenchymal stem cells can be differentiated into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, after continuous subculture and cryopreservation It still has multi-directional differentiation potential and can be used as...

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Problems solved by technology

[0004] (1) Stem cells are dependent on fetal bovine serum;

[0005] (2) Serum is susceptible to contamination by viruses, mycoplasma or other pathogens;

[0006] (3) Fetal bovine serum has adverse effects on the characteristics and differentiation of stem cells;

[0007] (4) For large-scale production, the existence of a large amount of serum increases the difficulty of purification in the later stage of production, increases the process flow, increases production costs, and reduces the recovery rate; some serum components are difficult to completely remove, which seriously affects the quality of the final product and poses a safety hazard

However, these media are expensive, and it is necessary to use auxiliary products to coat the cell culture bottles during the process of culturing MSCs

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