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ST cell low serum medium and preparation method thereof

A low serum and medium technology, applied in cell culture active agents, biochemical equipment and methods, germ cells, etc., can solve problems such as unfavorable ST cell culture, large quality differences, different serum origins and batch numbers, etc., to achieve cell growth. The effect of good state, strong targeting and stable cell growth state

Inactive Publication Date: 2016-10-12
TIANXINHE SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ST cells are cultivated by animal serum. Different animals have different places of origin and batch numbers, and the quality of each batch varies greatly, which is not conducive to the cultivation of ST cells.

Method used

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  • ST cell low serum medium and preparation method thereof
  • ST cell low serum medium and preparation method thereof
  • ST cell low serum medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Disclosed a kind of ST cell low serum culture medium in the embodiment 1, is made up of first component, second component, third component, fourth component and plant hydrolyzed protein, wherein plant hydrolyzed protein consists of 2.5 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0056] in:

[0057] The first component consists of ingredients as in Table 1:

[0058] The first group table in the embodiment 1 of table 1

[0059]

[0060]

[0061] The second component was included as shown in Table 2.

[0062] The second group table in the embodiment 1 of table 2

[0063]

[0064]

[0065] The third component is included as shown in Table 3.

[0066] The third group table in the embodiment 1 of table 3

[0067]

[0068]

[0069] The fourth component is included as shown in Table 4.

[0070] The fourth group table in the embodiment 1 ...

Embodiment 2

[0087] Disclosed a kind of ST cell low serum medium in the embodiment 2, is made up of the first component, the second component, the third component, the fourth component and plant hydrolyzate, wherein the plant hydrolyzate consists of 2 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0088] in:

[0089] The first component consists of ingredients as in Table 5:

[0090] The first group table in the embodiment 2 of table 5

[0091]

[0092]

[0093] The second component is included as shown in Table 6.

[0094] The second group table in the embodiment 2 of table 6

[0095]

[0096]

[0097] The third component is included as shown in Table 7.

[0098] The third group table in the embodiment 2 of table 7

[0099]

[0100]

[0101] The fourth component is included as shown in Table 8.

[0102] The fourth group table in the embodiment 2 of ta...

Embodiment 3

[0119] Disclosed a kind of ST cell low serum medium in the embodiment 3, is made up of the first component, the second component, the third component, the fourth component and plant hydrolyzate, wherein the plant hydrolyzate consists of 3 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0120] in:

[0121] The first component consists of ingredients as in Table 5:

[0122] The first group table in the embodiment 3 of table 9

[0123]

[0124]

[0125] The second component was included as shown in Table 10.

[0126] Second group table in table 10 embodiment 3

[0127] components

Content (parts by weight)

Sodium chloride

189

Anhydrous Sodium Dihydrogen Phosphate

1.4

Calcium Chloride Anhydrous

3

Calcium Nitrate Tetrahydrate

1.3

copper sulfate pentahydrate

0.00012

potassium chloride

24

...

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Abstract

The invention discloses an ST (swine testis) cell low serum medium, which comprises: 110-250 parts of amino acid or its salt, 210-280 parts of D-glucose, 120-240 parts of an inorganic salt, 1-2 parts of vitamin, 2-3 parts of vegetable protein hydrolysate, 0.002-0.003 part of trace element, 0.008-0.012 part of glutathione, 0.00004-0.00009 part of hydrocortisone, 0.01-0.03 part of hypoxanthine, 0.0006-0.0010 part of linoleic acid, 0.026-0.038 part of insulin, 0.005-0.01 part of phenol red, and 90-280 parts of HEPES. The medium provided by the invention has the characteristics of simple composition, relatively low cost, and stable quality, the preparation method of the medium is simple to operate and is easy to control, all the components are uniformly dispersed in the medium, and different batches of mediums have small differences.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a ST cell low-serum medium and a preparation method thereof. Background technique [0002] ST cells are pig testis (swine testis) cells, which grow adherently in vitro, proliferate slowly during the culture process, and the cells tend to clump during digestion and passage. ST cells are sensitive to various viruses, such as classical swine fever virus (CSFV), porcine parvovirus (PPV), pseudorabies virus, etc. At present, ST cells are cultured by animal serum. Different animals have different places of origin and batch numbers, and the quality of each batch varies greatly, which is not conducive to the cultivation of ST cells. [0003] ST cell low-serum medium refers to a synthetic medium that needs to be added with a small amount of serum to maintain the growth and reproduction of cells between cities in vitro. It is composed of a variety of amino acids, vitamins, trace elements, plan...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0608C12N2500/10C12N2500/12C12N2500/14C12N2500/22C12N2500/24C12N2500/32C12N2500/34C12N2500/36C12N2500/38C12N2500/40C12N2501/33C12N2501/39C12N2501/998C12N2501/999
Inventor 赵洪磊
Owner TIANXINHE SUZHOU BIOTECH CO LTD
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