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Marc-145 cell low-serum culture medium and preparation method thereof

A low-serum, culture medium technology, applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems affecting the stability and continuity of experimental production, cell growth dependence, etc., to achieve cell growth The effect of good and stable condition, wide application prospect and small difference

Inactive Publication Date: 2016-12-21
TIANXINHE SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these animal-derived components bring obvious problems. The large amount of serum causes cell growth to be highly dependent on it, and the components of serum are very complex. There are certain differences in the components of serum between batches, which will directly affect the experiment. Stability and continuity of production

Method used

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  • Marc-145 cell low-serum culture medium and preparation method thereof
  • Marc-145 cell low-serum culture medium and preparation method thereof
  • Marc-145 cell low-serum culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A kind of PK cell low serum medium is disclosed in embodiment 1, is made up of first component, second component, third component, fourth component and plant hydrolyzed protein, wherein plant hydrolyzed protein consists of 2.5 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0055] in:

[0056] The first component consists of ingredients as in Table 1:

[0057] The first group table in the embodiment 1 of table 1

[0058]

[0059]

[0060] The second component was included as shown in Table 2.

[0061] The second group table in the embodiment 1 of table 2

[0062] components

Content (parts by weight)

Sodium chloride

660

Anhydrous Sodium Dihydrogen Phosphate

11

Calcium Chloride Anhydrous

19

Calcium Nitrate Tetrahydrate

5

copper sulfate pentahydrate

0.00009

potassium chloride

47...

Embodiment 2

[0083] A kind of PK cell low serum culture medium is disclosed in embodiment 2, is made up of the first component, the second component, the third component, the fourth component and plant hydrolyzed protein, wherein the plant hydrolyzed protein consists of 2 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0084] in:

[0085] The first component consists of ingredients as in Table 5:

[0086] The first group table in the embodiment 2 of table 5

[0087]

[0088]

[0089] The second component is included as shown in Table 6.

[0090] The second group table in the embodiment 2 of table 6

[0091]

[0092]

[0093] The third component is included as shown in Table 7.

[0094] The third group table in the embodiment 2 of table 7

[0095]

[0096]

[0097] The fourth component is included as shown in Table 8.

[0098] The fourth group table in t...

Embodiment 3

[0115] A kind of PK cell low serum medium is disclosed in embodiment 3, is made up of the first component, the second component, the third component, the fourth component and plant hydrolyzed protein, wherein the plant hydrolyzed protein consists of 3 parts by weight of Composition of one or more of soybean protein hydrolyzate, wheat protein hydrolyzate, potato protein hydrolyzate and pea protein hydrolyzate.

[0116] in:

[0117] The first component consists of ingredients as in Table 9:

[0118] The first group table in the embodiment 3 of table 9

[0119]

[0120]

[0121] The second component was included as shown in Table 10.

[0122] Second group table in table 10 embodiment 3

[0123]

[0124]

[0125] The third component is included as shown in Table 11.

[0126] The third group table in table 11 embodiment 3

[0127]

[0128]

[0129] The fourth component is included as shown in Table 12.

[0130]The fourth group table in table 12 embodiment 3 ...

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Abstract

The invention discloses a Marc-145 cell low-serum culture medium. The marc-145 cell low-serum culture medium is prepared from the following components in parts by weight: 157-345 parts of amino acid or salt thereof, 300-580 parts of D-glucose, 390-850 parts of inorganic salt, 1.5-4.2 parts of vitamin, 2-3 parts of vegetable protein hydrolysate, 0.003-0.004 part of microelements, 0.003-0.010 part of glutathione, 0.00002-0.00005 part of hydrocortisone, 0.01-0.03 part of hypoxanthine, 0.0008-0012 part of linoleic acid, 0.0012-0.0027 part of insulin, 0.001-0.005 part of phenol red, and 100-300 parts of HEPES. The Marc-145 cell low-serum culture medium is simple in component, relatively low in relative cost and stable in quality, and the preparation method of the culture medium is easy to operate and easy to control.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a Marc-145 cell low-serum medium and a preparation method thereof. Background technique [0002] Marc-145 cells are derived from monkey kidney cells, which are epithelioid cells, cloned from mother cells (Ma-104 cells), and can be continuously subcultured. At present, the traditional culture method of Marc-145 cells is to adopt the most basic synthetic medium, or even add about 10% newborn bovine serum to the natural medium (such as hydrolyzed milk protein) for cultivation. However, these animal-derived components bring obvious problems. The large amount of serum causes cell growth to be highly dependent on it, and the components of serum are very complex. There are certain differences in the components of serum between batches, which will directly affect the experiment. Stability and continuity of production. Serum is derived from animals and may contain unknown sources of infectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0686C12N2500/10C12N2500/12C12N2500/14C12N2500/16C12N2500/22C12N2500/24C12N2500/30C12N2500/32C12N2500/34C12N2500/38C12N2500/40C12N2501/33C12N2501/39C12N2501/998C12N2501/999
Inventor 赵洪磊
Owner TIANXINHE SUZHOU BIOTECH CO LTD
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