Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Low serum medium applicable to culturing of LMHs cell adhering to walls and preparation method of low serum medium

An adherent culture, low-serum technology, applied in the field of low-serum culture medium and its preparation, can solve the problems of easy agglomeration growth, high production cost, difficulty in LMH cell adhesion, etc. Enhance the effect of the sticker effect

Pending Publication Date: 2020-08-11
SICHUAN BAINUOJI TECH CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned LMH cell attachment difficulties, easy to aggregate growth, very picky about the quality of serum, usually need to use DMEM with 10% fetal bovine serum to culture virus, high production cost and other defects, the present invention provides a method suitable for LMH cell attachment cultured in low serum medium and optimized for LMH cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low serum medium applicable to culturing of LMHs cell adhering to walls and preparation method of low serum medium
  • Low serum medium applicable to culturing of LMHs cell adhering to walls and preparation method of low serum medium
  • Low serum medium applicable to culturing of LMHs cell adhering to walls and preparation method of low serum medium

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] A preparation method of low-serum culture medium suitable for the adherent culture of LMH cells includes the following steps:

[0031] (1) Take a clean container and add Tween 80 to 10ml of absolute ethanol. Stir thoroughly until it is completely dissolved. Add vitamin D3, linoleic acid, linolenic acid, and vitamin E in sequence. After each substance is completely dissolved, add another A substance to obtain a lipid nutrient mixture;

[0032] (2) Take a clean container, add 600ml of ultrapure water, add manganese gluconate, fumaric acid, pyridoxal phosphate, chromium picolinate, and lipopeptide in sequence. After stirring for 8 minutes with a magnetic stirrer, add in sequence: oxaloacetic acid , Anhydrous sodium dihydrogen phosphate, calcium chloride, sodium chloride, potassium chloride, potassium dihydrogen phosphate, L arginine hydrochloride, aspartic acid, cystine dihydrochloride, glutamic acid, leucine Acid, isoleucine, methionine, phenylalanine, serine, threonine, tryp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a low serum medium applicable to culturing of LMHs cells adhering to walls and a preparation method of the low serum medium, and belongs to the field of biological products. Theculture medium includes fumaric acid, succinate, betaine, cysteamine, dextran, pyridoxine phosphate, beta-mercaptoacetic acid, chromium picolinate, carnitine hydrochloride, Glutamine-S, selenomethionine and other substances. The low serum medium applicable to culturing of the LMHs cells adhering to the walls prepared by the invention is clear in component, free of protein components, free of animal-derived components, low in blood serum dosage (2%-3%), and high in virus yield, can be used for preparing adenovirus of which the titer can reach 109TCID50 / ml, and can be used for culturing the LMHcells which grow uniformly and will not grow in clusters.

Description

Technical field [0001] The invention relates to the field of biological products, in particular to a low serum culture medium suitable for LMH cell adherence culture and a preparation method thereof. Background technique [0002] Since 2014, subgroup I avian adenovirus has been isolated in many areas of my country. The epidemic trend began in 2015. In 2017, the incidence of poultry decreased, and the incidence of waterfowl increased. However, since March 2018, the incidence of poultry has been on the rise, and it has become more and more serious, manifested as inclusion body hepatitis and hydropericardial syndrome, which have brought major harm to poultry breeding and food health. As a result, my country’s animal vaccine companies have begun to pay attention to avian adenovirus. The National Veterinary Drug Information Database shows that there are no biological products against avian adenovirus on the market, but the number of biological products that have been declared for cli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/30C12N2500/12C12N2500/14C12N2500/32C12N2500/34C12N2501/999C12N2500/44C12N2500/38C12N2500/35
Inventor 吴福文王永胜扶星星常其秀黄丽丁晓翠丁平萍
Owner SICHUAN BAINUOJI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com