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Method of preparing mink canine distemper antigen-protein composite, the antigen-protein composite and applications thereof

An antigen protein, canine distemper technology, applied in biochemical equipment and methods, viral antigen components, microorganisms, etc., can solve the problems of unstable quality, long complex cycle, high labor intensity, etc., and achieve easy amplification and cultivation, artificial reduction , the effect of improving safety

Active Publication Date: 2019-01-29
JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the cumbersome and complicated process of producing the mink distemper antigen protein complex, the long cycle, high labor intensity, laborious work, unstable quality, large batch-to-batch variation, and high side effects after the prepared virus antigen complex is injected into animals, etc. , the present invention provides a method for preparing mink distemper antigen protein complex by using low serum suspension culture VeroE6-TY strain cells, antigen protein complex and application thereof

Method used

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  • Method of preparing mink canine distemper antigen-protein composite, the antigen-protein composite and applications thereof
  • Method of preparing mink canine distemper antigen-protein composite, the antigen-protein composite and applications thereof
  • Method of preparing mink canine distemper antigen-protein composite, the antigen-protein composite and applications thereof

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Embodiment 1

[0048] 1. Recovery of VeroE6-TY strain cells

[0049] Take the VeroE6-TY strain suspension culture cells frozen in liquid nitrogen, place them in a 37°C water bath to thaw quickly and inoculate them in a shake flask containing 300ml of low serum suspension culture medium, place them at 37°C, 60rpm, 5% (v / v) CO 2 Cultured on a shaker for 72 hours, the cell density can reach 2.0×10 6 cells / ml, cell viability ≧90%, and recovered VeroE6-TY strain cells were obtained.

[0050] 2. Low-serum culture medium for amplified culture of VeroE6-TY strain cell culture medium

[0051] A) Inoculate the recovered VeroE6-TY strain cells obtained in step 1 into shake flasks (3 shake flasks) with low serum culture medium at a volume ratio of 1:3 to the culture medium, and supplement each bottle with low serum culture solution to 300ml at 37°C 5% CO 2 Cultivate on a shaker for 72 hours, 60r / min. The number of suspended cells is not less than 1.5×10 6 cells / ml, cell viability≧90%, obtained th...

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Abstract

The invention relates to a method of preparing a mink canine distemper antigen-protein composite, the antigen-protein composite and applications thereof and belongs to the field of bio-pharmacy. In the invention, mink canine distemper virus is cultured and domesticated by a low-serum culture medium with suspension of VeroE6-TY cell strain; then the domesticated virus is inoculated to a low-serum culture liquid with suspension of VeroE6-TY cell strain; then after cultivation and proliferation, a virus liquid is collected to obtain the mink canine distemper antigen-protein composite. For the mink canine distemper antigen-protein composite, conversion from spinner bottle adherent culture into large-scale cell suspension culture is achieved, so that production process is simplified and production cost is reduced; in addition, quality and yield of the product are greatly improved.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a preparation method of an antigen-protein complex, an antigen-protein complex and applications thereof. Background technique [0002] Mink distemper is an acute, highly contagious disease of mink caused by canine distemper virus. It mainly affects the respiratory system, digestive system and nervous system, and is characterized by typical bidirectional heat and catarrhal inflammation of the upper respiratory tract, lung and gastrointestinal tract. One of the three most harmful infectious diseases in fur animal breeding has brought huge economic losses to the breeding industry. VeroE6 cells are African green monkey kidney passage cells, which are widely used in the research of virus antigen preparation. Traditional VeroE6 cells are anchorage-dependent growth cells. When producing vaccines, 10 liters or 15 liters of spinner bottles are used to adhere to culture cell...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/175A61P31/14
CPCA61K39/12C12N7/00C12N2760/18434C12N2760/18451A61K2039/552
Inventor 任飞王春霞王彬叶明闫如勋刘秉玺李云松倪佳王萃瑜许皓
Owner JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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