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Vero cell low-serum culture medium and application thereof

A low serum and culture medium technology, applied in cell culture active agent, animal cells, culture process, etc., can solve the problems affecting the stability and quality of vaccine production process, the effect of downstream purification of cell growth, and the difference between serum batches, etc. Eliminate instability, reduce the complexity of purification process, and clarify the effect of ingredients

Active Publication Date: 2020-03-31
苏州依科赛生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many shortcomings in the use of serum: ① there are differences between serum batches, which affect the stability and quality of the vaccine production process; ② the use of serum is easy to cause microbial contamination; ③ the composition of serum is complex, and there are substances that promote cell growth and substances that inhibit cell growth , affecting cell growth and subsequent downstream purification; ④Serum is expensive
But about the present invention a kind of Vero cell low serum culture medium and application thereof also have no report

Method used

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  • Vero cell low-serum culture medium and application thereof
  • Vero cell low-serum culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Vero cell low serum medium (1)

[0035] Includes basal medium and supplementary components:

[0036] Added components: EDTA disodium 1mg / L, dihydrate ethanolamine hydrochloride 5mg / L, EDTA iron sodium 2mg / L, rhEGF 0.005mg / L, inositol 50mg / L, vitamin B60 .5mg / L, vitamin B70.01mg / L;

[0037] Basal medium: DMEM / F12.

Embodiment 2

[0038] Embodiment 2 Vero cell low serum culture medium (two)

[0039] Includes basal medium and supplementary components:

[0040] Added components: EDTA disodium 10mg / L, dihydrate ethanolamine hydrochloride 30mg / L, EDTA iron sodium 25mg / L, rhEGF 0.02mg / L, inositol 80mg / L, vitamin B65mg / L, vitamin B70.3mg / L;

[0041] Basal medium: DMEM / F12.

Embodiment 3

[0042] Example 3 Vero Cell Low Serum Medium (3)

[0043] Includes basal medium and supplementary components:

[0044] Added components: disodium EDTA 1mg / L, ethanolamine dihydrate hydrochloride 30mg / L, iron sodium EDTA 2mg / L, rhEGF 0.02mg / L, inositol 50mg / L, vitamin B65mg / L, vitamin B70.01mg / L;

[0045] Basal medium: DMEM / F12.

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Abstract

The invention relates to a Vero cell low-serum culture medium and an application thereof. The Vero cell low-serum culture medium comprises a basal culture medium and an additive component, wherein thebasal culture medium is prepared from the following components; DMEM / F12, the additive component is composed of the following components with the final concentration: 1 to 10mg / L of disodium ethylenediamine tetraacetate, 5 to 30 mg / L of dihydrate ethanolamine hydrochloride, 2 to 25 mg / L of ethylenediamine tetraacetic acid ferric sodium salt, 0.005 to 0.02 mg / L of recombinant epidermal growth factor (rhEGF), 50 to 80 mg / L of inositol, 0.5 to 5 mg / L of vitamin B6 and 0.01 to 0.3 mg / L of vitamin B7. The invention also comprises the application of the culture medium in Vero cell culture. The Vero cell culture medium has the advantages that Vero cells grow well in the culture medium, the production stability of biological products is improved, and the complexity degree and the production costof a later purification process are reduced.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a Vero cell low-serum culture medium and its application. Background technique [0002] Vero cell (African green kidney cell, Verocell) is a vaccine production cell line approved by the World Health Organization and my country's biological products regulations. It has been used in the production of various vaccines. Compared with primary cells, diploid cells and other subcultured cell substrates used for vaccine production, Vero cells have the following characteristics: ①The source is convenient, can be continuously passaged, and the growth rate is fast; ②It is sensitive to infection by various viruses, High virus proliferation titer; ③Stable genetic traits, low degree of malignant transformation, high biological safety; ④Cultivation conditions are not harsh, and it is easy to implement large-scale cultivation in bioreactors. [0003] Culture medium is the most direct and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N7/00C12R1/93
CPCC12N5/0686C12N7/00C12N2500/30C12N2500/35C12N2500/38C12N2500/46C12N2501/11C12N2760/20151C12N2770/24151C12N2770/32351
Inventor 徐亮亮陈旭陈刚
Owner 苏州依科赛生物科技股份有限公司
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