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Aconite fingerprint, its establishment method and aconite quality detection method

A technique of fingerprinting and establishing methods, which is applied in the field of drug testing and can solve the problem of single quality evaluation methods of aconite

Active Publication Date: 2022-03-08
YAAN THREE NINE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality evaluation method of aconite in the Chinese Pharmacopoeia is only single, which is not enough to systematically and completely indicate the overall quality of aconite and its preparations

Method used

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  • Aconite fingerprint, its establishment method and aconite quality detection method
  • Aconite fingerprint, its establishment method and aconite quality detection method
  • Aconite fingerprint, its establishment method and aconite quality detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0078] Prepare reference substance solution: adopt 50vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, so that every 1ml reference substance solution contains Formyl neoaconitine 10 μg, benzoyl aconitine 10 μg and benzoyl hypoaconitine 10 μg;

[0079] Prepare aconite need testing solution; get aconite powder, add 50vol% ethanol aqueous solution, close up, sonicate for 30min, filter, decompress and recover the solvent in the filtrate to dryness, then adopt 50vol% methanol aqueous solution to dissolve and settle to 5ml; aconite powder and The ratio of the aqueous ethanol solution was 0.04 g / ml.

[0080] Accurately draw 10 μl each of the reference substance solution and the test solution, respectively inject into the liquid chromatograph, measure, re...

Embodiment 2

[0087] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0088] Prepare reference substance solution: adopt 48vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, so that every 1ml reference substance solution contains Formyl neoaconitine 8 μg, benzoyl aconitine 8 μg and benzoyl aconitine 8 μg;

[0089] Prepare aconite test solution; get aconite powder, add 35vol% ethanol aqueous solution, plug, ultrasonically treat for 40min, filter, decompress and reclaim the solvent in the filtrate to dryness, then adopt 35vol% methanol aqueous solution to dissolve and settle to 4ml; aconite powder and The proportion of ethanol aqueous solution is 0.03g / ml;

[0090] Precisely draw 10 μl each of the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for deter...

Embodiment 3

[0097] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0098] Prepare reference substance solution: adopt 52vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, make every 1ml reference substance solution contain Formyl neoaconitine 9 μg, benzoyl aconitine 9 μg and benzoyl aconitine 9 μg;

[0099] Prepare aconite need testing solution; get aconite powder, add 45vol% ethanol aqueous solution, close up, ultrasonic treatment 40min, filter, decompress and recover the solvent in the filtrate to dryness, then adopt 45vol% methanol aqueous solution to dissolve and settle to 4ml; Aconite powder and The proportion of ethanol aqueous solution is 0.05g / ml;

[0100] Precisely draw 10 μl each of the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for d...

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Abstract

The invention relates to the technical field of drug detection, in particular to a fingerprint of aconite, a method for establishing the same and a method for detecting the quality of aconite. The establishment method of the method for fingerprinting of Fuzi mainly includes the following steps: preparing a reference substance solution, preparing aconite test solution; and measuring the Aconite test solution and the reference substance solution with high performance liquid chromatography. The method provided by the embodiment of the present invention is stable, reliable, simple in operation and good in repeatability. The parameters of the fingerprint analysis method provided by the invention can meet higher requirements in terms of precision, repeatability, durability and specificity, which proves that the method is stable and reliable.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a fingerprint of aconite, a method for establishing the same and a method for detecting the quality of aconite. Background technique [0002] Aconite, the name of traditional Chinese medicine. It is a processed product of the root of Aconitum aconitum of Ranunculaceae, also known as Fupian, Yanfuzi and Fuzi. At present, the quality evaluation method of aconite in the "Chinese Pharmacopoeia" is only single, which is not enough to systematically and completely indicate the overall quality of aconite and its preparations. Contents of the invention [0003] The purpose of the present invention is to provide an aconite fingerprint, its establishment method and aconite quality detection method, in order to accurately analyze and measure the aconite quality, can effectively and reliably predict product quality, and provide a basis for quality evaluation. [0004] A first aspe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 王丽婷董礼蒋晓凤韦婷
Owner YAAN THREE NINE PHARMA